Anti-PDPK1 Antibody Picoband®

Western blot analysis of PDPK1 using anti-PDPK1 antibody (PB9735).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Liver Tissue Lysate,
Lane 2: Rat Lung Tissue Lysate,
Lane 3: Mouse Liver Tissue Lysate,
Lane 4: Mouse Lung Tissue Lysate,
Lane 5: COLO320 Whole Cell Lysate,
Lane 6: MCF-7 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 antigen affinity purified polyclonal antibody (Catalog # PB9735) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDPK1 at approximately 70KD. The expected band size for PDPK1 is at 63KD.

BECCs regulate PI3K, PDPK1, and mTOR protein levels in HAPH rats. (A–C) Primitive bands of p-PI3K, PI3K, p-PDPK1, PDPK1, p-mTOR, and mTOR by Western blots in lung tissues. (D–F) Quantitative evaluation of p-PI3K, PI3K, p-PDPK1, PDPK1, p-mTOR, and mTOR in lung tissues. n = 5. All data are represented as the mean ± SD. * p < 0.05 vs. control group and # p < 0.05 vs. hypoxia group.
Index in PubMed under a CC BY license. PMID: 40385484

Flavonoids inhibit the proliferation of PASMCs under hypoxic conditions by inhibiting the PI3K/AKT axis. (A, B) Primitive bands and quantitative densities of p-AKT1 Ser473 and AKT1 with or without Sc79 (20 μM) by Western blots in PASMCs under 3% O 2 . (C, D) Primitive bands and quantitative densities of p-PI3K, PI3K, p-AKT1 Ser473, and AKT1 with or without 740Y-P (10 μM) by Western blots in PASMCs under 3% O 2 . (E, F) Primitive bands and quantitative densities of p-PDPK1, PDPK, p-AKT1 Ser473, and AKT1 with or without MYH1485 (2 μM) in PASMCs by Western blots under 3% O 2 . n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. 3% O 2 group, and p < 0.05 vs. 3% O 2 + Fla-50 μg/ml group.
Index in PubMed under a CC BY license. PMID: 40385484

IHC analysis of PDPK1 using anti-PDPK1 antibody (PB9735).
PDPK1 was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDPK1 Antibody (PB9735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IHC analysis of PDPK1 using anti-PDPK1 antibody (PB9735).
PDPK1 was detected in paraffin-embedded section of Rat Testis Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDPK1 Antibody (PB9735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IHC analysis of PDPK1 using anti-PDPK1 antibody (PB9735).
PDPK1 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDPK1 Antibody (PB9735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IHC analysis of PDPK1 using anti-PDPK1 antibody (PB9735).
PDPK1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDPK1 Antibody (PB9735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IHC analysis of PDPK1 using anti-PDPK1 antibody (PB9735).
PDPK1 was detected in frozen section of mouse small intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDPK1 Antibody (PB9735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.