Anti-ARID1A Antibody

Product Name

Anti-ARID1A Antibody

View all ARID1A Antibodies

SKU/Catalog Number

A00247

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ARID1A Antibody (Catalog # A00247). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ARID1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-ARID1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00247)

Host

Rabbit

Contents

ARID1A antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A. The immunogen is located within amino acids 580 - 630 of ARID1A.

Cross-reactivity

ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.

Reactive Species

A00247 is reactive to ARID1A in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

242.0 kDa

Background of ARID1A

The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00247 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ARID1A

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