Anti-DDR2 Antibody Picoband™

Boster Bio Anti-DDR2 Antibody Picoband™ catalog # A01698-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01698-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-DDR2 Antibody Picoband™

See all Ddr2 products

SKU/Catalog Number

A01698-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DDR2 Antibody Picoband™ catalog # A01698-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DDR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01698-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human DDR2 recombinant protein (Position: T801-E855).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01698-1 is reactive to DDR2 in Human, Mouse, Rat

Applications

A01698-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For DDR2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16832

Gene ID

4921

Gene Name

DDR2

Full Name

Discoidin domain-containing receptor 2

Weight

96.736kDa

Superfamily

protein kinase superfamily

Alternative Names

CD167 antigen-like family member B; CD167b antigen; DDR2; Discoidin domain receptor 2; discoidin domain receptor family, member 2; discoidin domain receptor tyrosine kinase 2; discoidin domain-containing receptor 2; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3cell migration-inducing protein 20; Receptor protein-tyrosine kinase TKT; TKT; TKTMIG20a; Trk3; TYRO10; Tyro-10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase DDR2 MIG20a, NTRKR3, TKT, TYRO10, WRCN discoidin domain receptor tyrosine kinase 2 discoidin domain-containing receptor 2|CD167 antigen-like family member B|cell migration-inducing protein 20|discoidin domain receptor 2|discoidin domain receptor family, member 2|discoidin domain-containing receptor tyrosine kinase 2|hydroxyaryl-protein kinase|migration-inducing gene 16 protein|neurotrophic tyrosine kinase receptor related 3|receptor protein-tyrosine kinase TKT|tyrosine-protein kinase TYRO10

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DDR2, check out the DDR2 Infographic

DDR2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DDR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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16 Customer Q&As for Anti-DDR2 Antibody Picoband™

Question

Our lab used your anti-DDR2 antibody for IHC on heart thymus in a previous project. I am using rat, and We intend to use the antibody for WB next. My question regards examining heart thymus as well as skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

I have checked the website and datasheets of our anti-DDR2 antibody and it appears that A01698-1 has been validated on rat in both IHC and WB. Thus A01698-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-24

Question

My question regards to test anti-DDR2 antibody A01698-1 on rat heart thymus for research purposes, then I may be interested in using anti-DDR2 antibody A01698-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Jones

Verified customer

Asked: 2020-02-05

Answer

The products we sell, including anti-DDR2 antibody A01698-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-05

Question

I was wanting to use your anti-DDR2 antibody for IHC for rat heart thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat heart thymus identification?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

You can see on the product datasheet, A01698-1 anti-DDR2 antibody has been validated for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat heart thymus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-14

Question

Do you have a BSA free version of anti-DDR2 antibody A01698-1 available?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DDR2 antibody A01698-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-06

Question

Does A01698-1 anti-DDR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

It shows on the product datasheet, A01698-1 anti-DDR2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-05

Question

I see that the anti-DDR2 antibody A01698-1 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-09

Question

Is a blocking peptide available for product anti-DDR2 antibody (A01698-1)?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

We do provide the blocking peptide for product anti-DDR2 antibody (A01698-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-04

Question

See attached the WB image, lot number and protocol we used for heart thymus using anti-DDR2 antibody A01698-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-12

Question

We are currently using anti-DDR2 antibody A01698-1 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

The anti-DDR2 antibody (A01698-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-15

Question

Is this A01698-1 anti-DDR2 antibody reactive to the isotypes of DDR2?

E. Zhao

Verified customer

Asked: 2019-05-01

Answer

The immunogen of A01698-1 anti-DDR2 antibody is E. coli-derived human DDR2 recombinant protein (Position: T801-E855). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-01

Question

We have observed staining in human hair follicle dermal papilla uterus. Any tips? Is anti-DDR2 antibody supposed to stain hair follicle dermal papilla uterus positively?

J. Lewis

Verified customer

Asked: 2019-03-04

Answer

From literature hair follicle dermal papilla uterus does express DDR2. From Uniprot.org, DDR2 is expressed in adipose tissue, heart thymus, hair follicle dermal papilla uterus, skin, cervix carcinoma, among other tissues. Regarding which tissues have DDR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Hair follicle dermal papilla, and Uterus, Pubmed ID: 14702039
Heart, and Thymus, Pubmed ID: 8247548
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-03-04

Question

My team were content with the WB result of your anti-DDR2 antibody. However we have observed positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is DDR2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-02-15

Answer

Based on literature, cervix carcinoma does express DDR2. Generally DDR2 expresses in cell membrane. Regarding which tissues have DDR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Hair follicle dermal papilla, and Uterus, Pubmed ID: 14702039
Heart, and Thymus, Pubmed ID: 8247548
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-02-15

Question

I am interested in using your anti-DDR2 antibody for spondyloepimetaphyseal dysplasia short limb-hand type studies. Has this antibody been tested with western blotting on mouse kidney tissue? We would like to see some validation images before ordering.

N. Mangal

Verified customer

Asked: 2018-04-13

Answer

I appreciate your inquiry. This A01698-1 anti-DDR2 antibody is validated on rat kidney tissue, mouse kidney tissue, small intestine tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-04-13

Question

My question regarding product A01698-1, anti-DDR2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-01-02

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01698-1 anti-DDR2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-02

Question

Does anti-DDR2 antibody A01698-1 work for IHC with heart thymus?

Verified Customer

Verified customer

Asked: 2017-11-02

Answer

According to the expression profile of heart thymus, DDR2 is highly expressed in heart thymus. So, it is likely that anti-DDR2 antibody A01698-1 will work for IHC with heart thymus.

Boster Scientific Support

Answered: 2017-11-02

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart thymus using anti-DDR2 antibody A01698-1. Let me know if you need anything else.

R. Williams

Verified customer

Asked: 2014-11-07

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-11-07

Conjugation

how to order through PO

Total: $280

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A01698-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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