TIMP1 antibody (PA1385) performs well in IHC, WB and shows reactivity with Human.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 14 Publications
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Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband®

TIMP1 antibody

Reactive SpeciesHostApplication
HumanRabbitIHC, WB

SKU: PA1385

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

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Tested Applications

TIMP1 antibody was positively detected by WB in:

human U-87MG whole cell, human U2OS whole cell, human PC-3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

TIMP1 antibody was positively detected by IHC in:

Human Mammary Cancer Tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Recommend Dilution

Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Western blot
0.1-0.5μg/ml
Human

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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WB
IHC
IF
Human
Tested
Tested
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Product Information

Product Name
Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband®
View all TIMP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP-1.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated Molecular Weight

23.2 kDa

Background of TIMP1
TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. TIMP was found to be located about 22 cM proximal to OTC (300461).
Cite This Product
Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1385)

Protocols

Western Blot protocol for TIMP1 antibody
Immunohistochemistry (IHC) protocol for TIMP1 antibody

Publications

Application:
Species:
Timosaponin BII inhibits TGF-β mediated epithelial-mesenchymal transition through Smad-dependent pathway during pulmonary fibrosis
Published: 2023-02-18
Journal: PHYTOTHERAPY RESEARCH
Application: IHC
Impact Factor: 7.20
Species: Rat
PMID: 36807664
Chlorogenic Acid Inhibits Liver Fibrosis by Blocking the miR-21-Regulated TGF-β1/Smad7 Signaling Pathway in Vitro and in Vivo
Published: 2017-12-19
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.40
Species: Human
PMID: 29311932
Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia
Published: 2015-01-20
Journal: Experimental and Therapeutic Medicine
Application: IHC
Impact Factor: 1.27
Species: Human
PMID: 25667666
Th17 Down-regulation Is Involved in Reduced Progression of Schistosomiasis Fibrosis in ICOSL KO Mice
Published: 2015-01-15
Journal: PLoS Neglected Tropical Diseases
Application: IHC
Impact Factor: 4.45
Species: Mouse
PMID: 25590646
Sanmiao formula inhibits chondrocyte apoptosis and cartilage matrix degradation in a rat model of osteoarthritis
Published: 2014-07-24
Journal: Experimental and Therapeutic Medicine
Application: WB
Impact Factor: 0.94
Species: Human
PMID: 25187798
Effect of focal mild hypothermia on expression of MMP-9, TIMP-1, Tau-1 and β-APP in rats with cerebral ischaemia/reperfusion injury
Published: 2013-08-08
Journal: BRAIN INJURY
Application: IHC
Impact Factor: 1.51
Species: Rat
PMID: 23895636
Anti-fibrotic effects of puerarin on CCl4-induced hepatic fibrosis in rats possibly through the regulation of PPAR-γ expression and inhibition of PI3K/Akt pathway
Published: 2013-03-13
Journal: FOOD AND CHEMICAL TOXICOLOGY
Impact Factor: 3.01
PMID: 23500774
The effect of 2,3,4′,5-tetrahydroxystilbene-2-0-β-d glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms
Published: 2012-11-21
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Impact Factor: 2.52
PMID: 23178522
Acipimox attenuates atherosclerosis and enhances plaque stability in ApoE-deficient mice fed a palmitate-rich diet
Published: 2012-10-08
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: IHC
Impact Factor: 2.48
Species: Mouse
PMID: 23058919
Synthetic gelatinases inhibitor attenuates electromagnetic pulse-induced blood–brain barrier disruption by inhibiting gelatinases-mediated ZO-1 degradation in rats
Published: 2011-04-08
Journal: TOXICOLOGY
Application: WB,IHC
Impact Factor: 3.64
Species: Rat
PMID: 21501651
Noninvasive Limb Ischemic Preconditioning Protects Against Myocardial I/R Injury in Rats
Published: 2009-04-16
Journal: JOURNAL OF SURGICAL RESEARCH
Application: IHC
Impact Factor: 1.88
Species: Rat
PMID: 19726056
Inhibition effect of Guizhi-Fuling-decoction on the invasion of human cervical cancer
Published: 2008-08-08
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 2.05
Species: Human,Mouse
PMID: 18761069
Effect of Danshao Huaxian capsule on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibrotic liver of rats
Published: 2005-08-28
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Impact Factor: 0.00
PMID: 16124044
Effects of augmentation of liver regeneration recombinant plasmid on rat hepatic fibrosis
Published: 2005-04-28
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Impact Factor: 0.00
PMID: 15832414

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1 Review For Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband®

Good TIMP-1 Antibody for Western Blotting--Swati Naphade, Aging, Buck Institute, Postdoctoral Fellow

Source: Biocompare.com

SKU PA1385
Application Western Blot
Sample Human NSC cell lysates
Detection ECL chemiluminescence

"We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. The antibody detects a band at 28kD, which is the predicted molecular weight of TIMP-1. Good antibody for TIMP-1, cleaner than other antibodies I have tested. In summary, this antibody is good for human samples and I highly recommend it."

Verified customer

Submitted

Product Promise

We promise Anti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
PA1385
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Sep 23, 2026