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Product Info Summary
| SKU: | M12041 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-DDX50 Mouse Monoclonal Antibody [Clone ID: OTI4F7]
SKU/Catalog Number
M12041
Size
100 µl
Description
Boster Bio DDX50 mouse monoclonal antibody,clone OTI4F7. Catalog# M12041. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-DDX50 Mouse Monoclonal Antibody [Clone ID: OTI4F7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12041)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI4F7
Isotype
IgG2b
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-287 of human DDX50 (NP_076950) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M12041 is reactive to DDX50 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
82.565kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12041 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX50 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX50.
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Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-DDX50 monoclonal antibody (1:500).
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-DDX50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDX50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-DDX50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDX50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Protein Target Info & Infographic
Gene/Protein Information For DDX50 (Source: Uniprot.org, NCBI)
Gene Name
DDX50
Full Name
ATP-dependent RNA helicase DDX50
Weight
82.565kDa
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase DDX50; DEAD (Asp-Glu-Ala-Asp) box polypeptide 50; DEAD box protein 50; EC 3.6.1; EC 3.6.4.13; GU2; GUB; gu-beta; MGC3199; Nucleolar protein Gu2; RH-II/GuB; RNA helicase II/Gu beta DDX50 GU2, GUB, RH-II/GuB, mcdrh DExD-box helicase 50 ATP-dependent RNA helicase DDX50|DEAD (Asp-Glu-Ala-Asp) box polypeptide 50|DEAD box protein 50|DEAD-box helicase 50|RNA helicase II/Gu beta|gu-beta|malignant cell derived RNA helicase|nucleolar protein GU2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDX50, check out the DDX50 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDX50: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX50 Mouse Monoclonal Antibody [Clone ID: OTI4F7] (M12041)
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