Anti-DDX52 Antibody

DDX52 antibody

Boster Bio Anti-DDX52 Antibody catalog # A12204. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A12204
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-DDX52 Antibody

View all DDX52 Antibodies

SKU/Catalog Number

A12204

Size

100μl

Form

Liquid

Description

Boster Bio Anti-DDX52 Antibody catalog # A12204. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DDX52 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12204)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

F7E4

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human DDX52. at AA rangle: 530-610

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12204 is reactive to DDX52 in Human

Applications

A12204 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

67.498kDa

Background of DDX52

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For DDX52 (Source: Uniprot.org, NCBI)

Gene Name

DDX52

Full Name

Probable ATP-dependent RNA helicase DDX52

Weight

67.498kDa

Superfamily

DEAD box helicase family

Alternative Names

ATP-dependent RNA helicase ROK1-like; DEAD (Asp-Glu-Ala-Asp) box polypeptide 52; DEAD box protein 52; EC 3.6.1; HUSSY19; probable ATP-dependent RNA helicase DDX52; ROK1EC 3.6.4.13 DDX52 HUSSY19, ROK1 DExD-box helicase 52 probable ATP-dependent RNA helicase DDX52|DEAD (Asp-Glu-Ala-Asp) box polypeptide 52|DEAD box protein 52|DEAD-box helicase 52

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DDX52, check out the DDX52 Infographic

DDX52 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DDX52: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-DDX52 Antibody

Question

I see that the anti-DDX52 antibody A12204 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-15

Question

We are currently using anti-DDX52 antibody A12204 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?

K. Kulkarni

Verified customer

Asked: 2016-05-09

Answer

The anti-DDX52 antibody (A12204) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-05-09

Question

I was wanting to use your anti-DDX52 antibody for WB for human liver spleen on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver spleen identification?

F. Krishna

Verified customer

Asked: 2015-07-27

Answer

It shows on the product datasheet, A12204 anti-DDX52 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver spleen in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-07-27

Order DetailsPrice
A12204

100uL

$399

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