Human SEMA4G ELISA Kit PicoKine™

Human SEMA4G ELISA Kit PicoKine™ (96 Tests). Quantitate Human SEMA4G in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK2006
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human SEMA4G ELISA Kit PicoKine™

See all Semaphorin 4G products

SKU/Catalog Number

EK2006

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human SEMA4G ELISA Kit PicoKine™ (96 Tests). Quantitate Human SEMA4G in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human SEMA4G ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2006)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: V18-L675

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2006 is reactive to SEMA4G in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2006 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2006-CAP Anti-Human SEMA4G Pre-coated 96-well strip microplate 1
EK2006-ST Human SEMA4G Standard 2 vials, 10 ng/tube
EK2006-DA Human SEMA4G Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0460.1140.1800.3060.5170.8831.6402.358

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human SEMA4G ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2710246829114488
Standard deviation1.245.1024.341.486.7331.23
CV (%)4.6%5.0%5.2%5.1%5.9%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Semaphorin 4G in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 127232424241.506.2%
Sample 2102981041021012.172.1%
Sample 346853749050650025.145.0%
*number of samples for each test n=16.

Gene/Protein Information For SEMA4G (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NTN9

Gene ID

57715

Gene Name

SEMA4G

Full Name

Semaphorin-4G

Weight

91.497kDa

Superfamily

semaphorin family

Alternative Names

KIAA1619FLJ20590; MGC102867; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4G; SEMA4G; Semaphorin 4G; semaphorin-4G SEMA4G semaphorin 4G semaphorin-4G|sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SEMA4G, check out the SEMA4G Infographic

SEMA4G infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SEMA4G: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK2006

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5 Customer Q&As for Human SEMA4G ELISA Kit PicoKine™

Question

Q: how to proceed with the analysis of ELISA data? I have obtained Semaphorin 4G level in plasma.

Verified Customer

Verified customer

Asked: 2020-10-10

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-10-10

Question

Q: Can Semaphorin 4G ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-03-07

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-03-07

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Semaphorin 4G using the EK2006 Human Semaphorin 4G Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-05-18

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-05-18

Question

Q: What is the optimal O.D. value for Semaphorin 4G ELISA kit? I used your Semaphorin 4G ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Semaphorin 4G even though the O.D. values are not very high?

K. Green

Verified customer

Asked: 2017-10-09

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Semaphorin 4G ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Semaphorin 4G that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2017-10-09

Question

Q: if the enzyme conjugated Semaphorin 4G antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Semaphorin 4G antigen?

W. Brown

Verified customer

Asked: 2017-07-19

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-07-19

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