Rabbit IgG polyclonal antibody for Dihydropyrimidine dehydrogenase[NADP(+)](DPYD) detection. Tested with WB in Human;Mouse;Rat.
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-DPYD Antibody
See all DPYD primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for DPYD detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. DPYD information: Molecular Weight: 111401 MW; Subcellular Localization: Cytoplasm; Tissue Specificity: Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.|
|Cite This Product||Anti-DPYD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1761)|
|Specificity||Anti-DPYD Antibody (PA1761) reacts with Human, Mouse, Rat DPYD, in native form and recombinant. Superfamily members of DPYD are not reactive to PA1761.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human DPYD(33-52aa AKKLDKKHWKRNPDKNCFNC), different from the related rat and mouse sequences by one amino acid.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-DPYD Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-DPYD Antibody (PA1761).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Anti-DPYD antibody, PA1761, Western blotting
WB: MM231 Cell Lysate
Protein Target Info (Source: Uniprot.org)
|Protein Name||Dihydropyrimidine dehydrogenase [NADP(+)]|
|Tissue Specificity||Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.|
|Alternative Names||Dihydropyrimidine dehydrogenase [NADP(+)];DHPDHase;DPD;126.96.36.199;Dihydrothymine dehydrogenase;Dihydrouracil dehydrogenase;DPYD;|
|Molecular Weight||111401 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||DPYD(Dihydropyrimidine Dehydrogenase), also called DPD, is an enzyme that in humans is encoded by the DPYD gene. The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. The structure of the DPYD gene contains 23 exons spanning about 950 kb. Using somatic cell hybrid strategies, the DPYD gene is mapped to the centromeric region of chromosome 1 between 1p22 and 1q21. By fluorescence in situ hybridization, the DPYD gene is mapped to 1p22. The highest level of DPD was found in monocytes followed by that in lymphocytes, granulocytes, and platelets, whereas no significant activity of DPD could be detected in erythrocytes. The activity of DPD in peripheral blood mononuclear cells was intermediate between that observed in monocytes and lymphocytes. By cDNA microarray, Western blot analysis, and luciferase reporter assay, the transcription factor LSF was identified as a positive regulator of DPYD.|
Other Recommended Resources
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.