Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband™

Product Name

Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband™

View all Cytochrome P450 2B6 Antibodies

SKU/Catalog Number

A00861-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband™ catalog # A00861-2. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00861-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Cytochrome P450 2B6/CYP2B6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00861-2 is reactive to CYP2B6 in Human

Applications

A00861-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

56.278kDa

Background of Cytochrome P450 2B6

Cytochrome P450 2B6 is an enzyme that in humans is encoded by the CYP2B6 gene. It is mapped to 19q13.2. This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry, 1-3μg/1x10⁶ cells

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CYP2B6 using anti-CYP2B6 antibody (A00861-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2B6 antigen affinity purified polyclonal antibody (Catalog # A00861-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2B6 at approximately 56KD. The expected band size for CYP2B6 is at 56KD.

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Figure 2. IF analysis of Cytochrome P450 2B6/CYP2B6 using anti-Cytochrome P450 2B6/CYP2B6antibody (A00861-2).
Cytochrome P450 2B6/CYP2B6was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Cytochrome P450 2B6/CYP2B6 Antibody (A00861-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of THP-1 cells using anti-Cytochrome P450 2B6/CYP2B6 antibody (A00861-2).
Overlay histogram showing THP-1 cells stained with A00861-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytochrome P450 2B6/CYP2B6Antibody (A00861-2, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CYP2B6 (Source: Uniprot.org, NCBI)

Alternative Names

CPB6; CYP2B; CYP2B7; CYP2B7P; CYPIIB6cytochrome P450, subfamily IIB (phenobarbital-inducible); cytochrome P450 2B6; Cytochrome P450 IIB1; cytochrome P450, family 2, subfamily B; cytochrome P450, family 2, subfamily B, polypeptide 6; cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6; EC 1.14.14.1; IIB1; P450 CYP2B6 CPB6, CYP2B, CYP2B7, CYP2B7P, CYPIIB6, EFVM, IIB1, P450 cytochrome P450 family 2 subfamily B member 6 cytochrome P450 2B6|1,4-cineole 2-exo-monooxygenase|cytochrome P450 IIB1|cytochrome P450, family 2, subfamily B, polypeptide 6|cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6

For more info on CYP2B6, check out the CYP2B6 Infographic

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In this infographic, you will see the following information for CYP2B6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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