Anti-Dyskerin DKC1 Antibody

DKC1 antibody

Boster Bio Anti-Dyskerin DKC1 Antibody (Catalog # A01535-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01535-1
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, WB

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Product Name

Anti-Dyskerin DKC1 Antibody

View all DKC1 Antibodies

SKU/Catalog Number

A01535-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Dyskerin DKC1 Antibody (Catalog # A01535-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Dyskerin DKC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01535-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from Internal of human Dyskerin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01535-1 is reactive to DKC1 in Human, Mouse, Rat

Applications

A01535-1 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

57.674kDa

Background of DKC1

Isoform 1:Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.Isoform 3:Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).

Heiss N.S., Nat. Genet. 19:32-38(1998).
Knight S.W., Am. J. Hum. Genet. 65:50-58(1999).
Hassock S., Genomics 55:21-27(1999).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For DKC1 (Source: Uniprot.org, NCBI)

Gene Name

DKC1

Full Name

H/ACA ribonucleoprotein complex subunit DKC1

Weight

57.674kDa

Superfamily

pseudouridine synthase TruB family

Alternative Names

CBF5 homolog; CBF5; cbf5p homolog; DKC; dyskeratosis congenita 1, dyskerin; Dyskerin; EC 5.4.99; EC 5.4.99.-; FLJ97620; H/ACA ribonucleoprotein complex subunit 4; NAP57; NOLA4dyskerin; Nopp140-associated protein of 57 kDa; Nucleolar protein family A member 4; Nucleolar protein NAP57; snoRNP protein DKC1; XAP101 DKC1 CBF5, DKC, DKCX, NAP57, NOLA4, XAP101 dyskerin pseudouridine synthase 1 H/ACA ribonucleoprotein complex subunit DKC1|CBF5 homolog|H/ACA ribonucleoprotein complex subunit 4|dyskeratosis congenita 1, dyskerin|nopp140-associated protein of 57 kDa|nucleolar protein NAP57|nucleolar protein family A member 4|snoRNP protein DKC1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DKC1, check out the DKC1 Infographic

DKC1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DKC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Dyskerin DKC1 Antibody

Question

Does anti-Dyskerin antibody A01535-1 work on canine WB with layer of synovial tissue?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

Our lab technicians have not tested anti-Dyskerin antibody A01535-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Dyskerin antibody A01535-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine layer of synovial tissue in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-07

Question

I was wanting to use to test anti-Dyskerin antibody A01535-1 on human uterus for research purposes, then I may be interested in using anti-Dyskerin antibody A01535-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

The products we sell, including anti-Dyskerin antibody A01535-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-25

Question

I was wanting to use your anti-Dyskerin antibody for WB for human uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human uterus identification?

G. Edwards

Verified customer

Asked: 2019-02-08

Answer

As indicated on the product datasheet, A01535-1 anti-Dyskerin antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-08

Question

Does anti-Dyskerin antibody A01535-1 work for WB with uterus?

Verified Customer

Verified customer

Asked: 2018-10-31

Answer

According to the expression profile of uterus, DKC1 is highly expressed in uterus. So, it is likely that anti-Dyskerin antibody A01535-1 will work for WB with uterus.

Boster Scientific Support

Answered: 2018-10-31

Question

We are currently using anti-Dyskerin antibody A01535-1 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

A. Wu

Verified customer

Asked: 2017-12-15

Answer

The anti-Dyskerin antibody (A01535-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-15

Question

Is this A01535-1 anti-Dyskerin antibody reactive to the isotypes of DKC1?

Verified Customer

Verified customer

Asked: 2017-12-04

Answer

The immunogen of A01535-1 anti-Dyskerin antibody is Synthesized peptide derived from Internal of human Dyskerin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-04

Question

Would A01535-1 anti-Dyskerin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-06-01

Answer

It shows on the product datasheet, A01535-1 anti-Dyskerin antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-06-01

Order DetailsPrice
A01535-1-100uL

100uL

$360

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