Anti-E3 SUMO-protein ligase CBX4 CBX4 Antibody

Product Name

Anti-E3 SUMO-protein ligase CBX4 CBX4 Antibody

View all CBX4 Antibodies

SKU/Catalog Number

A03388

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-E3 SUMO-protein ligase CBX4 CBX4 Antibody (Catalog # A03388). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CBX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-E3 SUMO-protein ligase CBX4 CBX4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03388)

Host

Rabbit

Contents

CBX4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CBX4 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CBX4. The immunogen is located within amino acids 50 - 100 of CBX4.

Cross-reactivity

Two alternatively spliced transcript variants have been observed.

Reactive Species

A03388 is reactive to CBX4 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

61.4 kDa

Background of CBX4

Polycomb group (PcG) proteins are chromatin-associated proteins which are important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1 and RING domain-containing proteins (RING1, RING2 and BMI1). CBX4 (Chromobox protein homolog 4 or PC2), which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. It is a E3 SUMO-protein ligase which facilitates SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation. CBX4 is directly involved in the cellular response to DNA damage.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03388 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CBX4 antibody can be used for detection of CBX4 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CBX4

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