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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Mouse ACE2 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse ACE2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ace2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse ACE2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1188)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: CHO; Immunogen sequence: Q18-T740
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1188 is reactive to Ace2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK1188 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1188-CAP||Anti-Mouse Ace2 Pre-coated 96-well strip microplate||1|
|EK1188-ST||Mouse Ace2 Standard||2 vials, 10 ng/tube|
|EK1188-DA||Mouse Ace2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse ACE2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect ACE-2, Dilution ratio of 1:1, concentration in serum is 0.6-1ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse ACE2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of ACE-2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ace2 (Source: Uniprot.Org, NCBI)
Angiotensin-converting enzyme 2
peptidase M2 family
ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 18.104.22.168; Metalloprotease MPROT15 Ace2|2010305L05Rik|angiotensin I converting enzyme (peptidyl-dipeptidase A) 2|angiotensin-converting enzyme 2|ACE-related carboxypeptidase|angiotensin I converting enzyme 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ace2, check out the Ace2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ace2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1188 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gu J,Yin J,Zhang M,Li J,Wu Y,Chen J,Miao H.Study on the Clinical Significance of ACE2 and Its Age-Related Expression.J Inflamm Res.2021;14:2873-2882https://doi. org/10.2147/JIR.S315981
EK1188 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Chiang MH, Liang CJ, Liu CW, Pan BJ, Chen WP, Yang YF, Lee IT, Tsai JS, Lee CW, Chen YL. Front Pharmacol. 2017 Nov 14;8:819. doi: 10.3389/fphar.2017.00819. eCollection 2017. Aliskiren Improves Ischemia- and Oxygen Glucose Deprivation-Induced Cardi...
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