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Product Info Summary
| SKU: | EK1188 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Mouse ACE2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1188
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
ACE2 is commonly studied in renin–angiotensin system biology and epithelial/endothelial homeostasis, and is also used as a receptor-context readout in respiratory virology models (putative here due to page access failure). Assay context: sandwich ELISA to quantify Mouse ACE2 in cell culture supernatants and serum/plasma (heparin), sensitivity 10 pg/mL; ACE2-centric inflammation/remodeling studies are often contextualized with cytokine localization such as IL1A (putative) and matrix output markers such as COL1A1 (putative fibrosis/remodeling adjacency), with quantitative verification workflows sometimes routed through ELISA testing (method-context).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse ACE2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1188)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q18-T740
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1188 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1188 is reactive to Ace2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1188 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ace2
AngiotensinI-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1188-CAP | Anti-Mouse Ace2 Pre-coated 96-well strip microplate | 1 |
| EK1188-ST | Mouse Ace2 Standard | 2 vials, 10 ng/tube |
| EK1188-DA | Mouse Ace2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.061 | 0.161 | 0.244 | 0.428 | 0.737 | 1.179 | 1.792 | 2.225 |
Data Example Images
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Mouse ACE2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Ace2, Dilution ratio of 1:1, concentration in serum and plasma is 2.7-10.8 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse ACE2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 146 | 538 | 1252 | 131 | 557 | 1192 |
| Standard deviation | 11.38 | 27.88 | 75.77 | 10.48 | 31.74 | 84.63 |
| CV (%) | 7.8% | 4.7% | 6.1% | 8% | 5.7% | 7.1% |
Specific Publications For Mouse ACE2 ELISA Kit PicoKine® (EK1188)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Mouse ACE2 ELISA Kit PicoKine®
Question
Have you ever tested EK1188 with plasma collected with EDTA?
Verified customer
Asked: 2020-06-05
Answer
The Mouse ACE2 ELISA Kit PicoKine™ (EK1188) is a zinc metalloprotease of the ACE family and EDTA plasma is not recommended for use in this assay due to their chelating properties.
Boster Scientific Support
Answered: 2020-06-05
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
W. Li
Verified customer
Asked: 2019-04-20
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-04-20
Question
Q: how can I thaw whole blood sample for ACE-2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test ACE-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-03-22
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of ACE-2 in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the ACE-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/elisa-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-18
