Mouse CD23/FCER2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

CD23/Fc epsilon RII ELISA antibody pairs, Mouse, for 5 plates

Mouse CD23/FCER2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Fcer2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml.

Product Info Summary

SKU: EZ0924
Size: For 5 plates, 96 wells each
Reactive Species: Mouse
Application: ELISA

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Product Name

Mouse CD23/FCER2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all CD23/Fc epsilon RII products

SKU/Catalog Number

EZ0924

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Mouse CD23/FCER2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Fcer2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CD23/FCER2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0924)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E50-P331

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0924 is reactive to Fcer2 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EZ0924 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Storage of opened/reconstituted material
EZ0942-CA Rabbit anti- mouse Fcer2 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0942-DA Biotinylated goat anti- mouse Fcer2 polyclonal antibody (Detection Antibody) 500 µl, 13 µg/mL (recommended dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)
EZ0942-ST Lyophilized recombinant mouse Fcer2 standard 10 ng/tube×3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0210.0450.1370.2790.5371.0091.5032.141

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4851882843059723479105
Standard deviation24.25105.4522.6642.39185.41783.03
CV (%)5%5.6%6.2%7.1%7.9%8.6%

Gene/Protein Information For Fcer2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20693

Gene ID

Gene Name

Fcer2

Full Name

Low affinity immunoglobulin epsilon Fc receptor

Weight

37.648kDa

Alternative Names

BLAST-2; CD23; CD23A; CD23CD23 antigen; CLEC4J; CLEC4JC-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon RII; Fc fragment of IgE, low affinity II, receptor for (CD23); FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A); fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; low affinity immunoglobulin epsilon Fc receptor; Ly-42; Lymphocyte IgE receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Fcer2, check out the Fcer2 Infographic

Fcer2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Fcer2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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