Product Info Summary
SKU: | A03095-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-eIF4A3 Antibody
SKU/Catalog Number
A03095-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-eIF4A3 Antibody catalog # A03095-1. Tested in WB,ICC,IF,IHC,Flow Cytometry,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03095-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human SPON1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03095-1 is reactive to EIF4A3 in Human, Mouse, Rat
Applications
A03095-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
46.871kDa
Background of EIF4A3
Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.
McGuinness B.E., PLoS Biol. 3:433-449(2005).
Kitajima T.S., Curr. Biol. 15:353-359(2005).
Tang Z., Proc. Natl. Acad. Sci. U.S.A. 101:18012-18017(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of EIF4A3 using Anti-eIF4A3 Antibody (A03095-1).
ICC staining eIF4A3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-eIF4A3 Antibody A03095-1
Western blot analysis of eIF4A3 on SiHa cell lysates using anti-eIF4A3 at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EIF4A3 (Source: Uniprot.org, NCBI)
Gene Name
EIF4A3
Full Name
Eukaryotic initiation factor 4A-III
Weight
46.871kDa
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; DKFZp686O16189; EC 3.6.1; EC 3.6.4.13; EIF4AIII; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A; eukaryotic translation initiation factor 4A3; hNMP 265; KIAA0111eukaryotic translation initiation factor 4A, isoform 3; MGC10862; NMP 265; NMP265; Nuclear matrix protein 265; NUK34 EIF4A3 DDX48, Fal1, MUK34, NMP265, NUK34, RCPS, eIF-4A-III, eIF4A-III, eIF4AIII eukaryotic translation initiation factor 4A3 eukaryotic initiation factor 4A-III|ATP-dependent RNA helicase DDX48|ATP-dependent RNA helicase eIF4A-3|DEAD (Asp-Glu-Ala-Asp) box polypeptide 48|DEAD box protein 48|NMP 265|eukaryotic initiation factor 4A-like NUK-34|nuclear matrix protein 265
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4A3, check out the EIF4A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF4A3 Antibody (A03095-1)
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No publications found for A03095-1
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