Anti-eIF4B(Ab-422) Antibody

Boster Bio Anti-eIF4B (Ab-422) Antibody catalog # A00846. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00846
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-eIF4B(Ab-422) Antibody

See all EIF4B products

SKU/Catalog Number

A00846

Size

100μl

Form

Liquid

Description

Boster Bio Anti-eIF4B (Ab-422) Antibody catalog # A00846. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-eIF4B(Ab-422) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00846)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Peptide sequence around aa.420~424 (T-G-S-E-S) derived from Human eIF4B.

Reactive Species

A00846 is reactive to EIF4B in Human, Mouse, Rat

Applications

A00846 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:1000
IHC, 1:50-1:100

Validation Images & Assay Conditions

Gene/Protein Information For EIF4B (Source: Uniprot.Org, NCBI)

Uniprot ID

P23588

Gene ID

1975

Gene Name

EIF4B

Full Name

Eukaryotic translation initiation factor 4B

Weight

69.151kDa

Alternative Names

Eif4a2; eIF4B; EIF-4B; eukaryotic translation initiation factor 4B; PRO1843 EIF4B EIF-4B, PRO1843 eukaryotic translation initiation factor 4B eukaryotic translation initiation factor 4B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EIF4B, check out the EIF4B Infographic

EIF4B infographic

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In this infographic you will see the following information for EIF4B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-eIF4B(Ab-422) Antibody

Question

Would anti-eIF4B(Ab-422) antibody A00846 work for IHC with female gonad?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

According to the expression profile of female gonad, EIF4B is highly expressed in female gonad. So, it is likely that anti-eIF4B(Ab-422) antibody A00846 will work for IHC with female gonad.

Boster Scientific Support

Answered: 2020-01-03

Question

Will anti-eIF4B(Ab-422) antibody A00846 work on goat IHC with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-09-09

Answer

Our lab technicians have not validated anti-eIF4B(Ab-422) antibody A00846 on goat. You can run a BLAST between goat and the immunogen sequence of anti-eIF4B(Ab-422) antibody A00846 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-09

Question

Can you help my question with product A00846, anti-eIF4B(Ab-422) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00846 anti-eIF4B(Ab-422) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-09

Question

I was wanting to use your anti-eIF4B(Ab-422) antibody for IHC for rat female gonad on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat female gonad identification?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

You can see on the product datasheet, A00846 anti-eIF4B(Ab-422) antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat female gonad in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-04

Question

Does A00846 anti-eIF4B(Ab-422) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

You can see on the product datasheet, A00846 anti-eIF4B(Ab-422) antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-06

Question

We are currently using anti-eIF4B(Ab-422) antibody A00846 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

A. Moore

Verified customer

Asked: 2018-05-04

Answer

The anti-eIF4B(Ab-422) antibody (A00846) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-04

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A00846
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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