|Validated Species:||Bovine, Human|
Data & Images
|Product Name||Anti-Elastin Antibody (Monoclonal, BA-4)|
|Description||Mouse IgG monoclonal antibody for Elastin, elastin (ELN ) detection. Tested with IHC-P in Human; bovine. No cross reactivity with otherproteins.|
|Cite This Product||Anti-Elastin Antibody (Monoclonal, BA-4) BA-4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1038)|
|Replacement Item||This antibody may replace the following items: sc-107533|sc-292227|sc-365260|sc-376390|sc-374494 from Santa Cruz Biotechnology.|
|Validated Species||Bovine, Human|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||68469 MW|
|Protein Function||Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity). .|
|Tissue Specificity||Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin. .|
|Sequence Similarities||Belongs to the elastin family.|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix . Extracellular matrix of elastic fibers. .|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|elastin| cardiovascular|vasculature|vasculature markers|arterial|smooth muscle cell (smc)||
Background for Elastin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Elastin Antibody (Monoclonal, BA-4) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.25-0.5μg/ml, Bovine, human, By Heat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.