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Product Info Summary
| SKU: | M04387-3 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-elF2 alpha (EIF2S1) Mouse Monoclonal Antibody [Clone ID: OTI3H7]
SKU/Catalog Number
M04387-3
Size
100 µl
Description
Boster Bio Anti-EIF2S1 mouse monoclonal antibody, clone OTI3H7 (formerly 3H7). Catalog# M04387-3. Tested in FC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-elF2 alpha (EIF2S1) Mouse Monoclonal Antibody [Clone ID: OTI3H7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04387-3)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3H7
Isotype
IgG1
Immunogen
Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
Reactive Species
M04387-3 is reactive to EIF2S1 in Dog, Human, Monkey, Mouse, Rat
Calculated molecular weight
36.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04387-3 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000~2000
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody.
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HEK293T cells transfected with either EIF2S1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EIF2S1 antibody (M04387-3)
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Flow cytometric Analysis of Jurkat cells
Specific Publications For Anti-elF2 alpha (EIF2S1) Mouse Monoclonal Antibody [Clone ID: OTI3H7] (M04387-3)
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