Product Info Summary
SKU: | PB9011 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Adiponectin/ADIPOQ Antibody Picoband™
View all Adiponectin/Acrp30 Antibodies
SKU/Catalog Number
PB9011
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Adiponectin/ADIPOQ Antibody Picoband™ catalog # PB9011. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Adiponectin/ADIPOQ Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9011)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat Adiponectin recombinant protein (Position: I19-N244). Rat Adiponectin shares 90% amino acid (aa) sequence identity with mouse Adiponectin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9011 is reactive to ADIPOQ in Mouse, Rat
Applications
PB9011 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
26 kDa
Calculated molecular weight
26.414kDa
Background of Adiponectin/Acrp30
Adiponectin, also known as APM1 or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. It is mapped to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, it has been showed that Adiponectin is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, it has been found that links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. This gene is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011).
Adiponectin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011).
Adiponectin was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011).
Adiponectin was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011).
Adiponectin was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011).
Adiponectin was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. Western blot analysis of Adiponectin using anti-Adiponectin antibody (PB9011).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Testis Tissue Lysate,
Lane 2: Rat Spleen Tissue Lysate,
Lane 3: Rat Skeletal Muscle Tissue Lysate,
Lane 4: Rat Cardiac Muscle Tissue Lysate,
Lane 5: Rat Liver Tissue Lysate,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adiponectin antigen affinity purified polyclonal antibody (Catalog # PB9011) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adiponectin at approximately 26KD. The expected band size for Adiponectin is at 26KD.
Protein Target Info & Infographic
Gene/Protein Information For ADIPOQ (Source: Uniprot.org, NCBI)
Gene Name
ADIPOQ
Full Name
Adiponectin
Weight
26.414kDa
Alternative Names
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein ADIPOQ ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28 adiponectin, C1Q and collagen domain containing adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement-related 30 kDa protein|adipose most abundant gene transcript 1 protein|adipose specific collagen-like factor|gelatin-binding protein 28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADIPOQ, check out the ADIPOQ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADIPOQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Adiponectin/ADIPOQ Antibody Picoband™ (PB9011)
Hello CJ!
PB9011 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang R, Xie X, Yu Q, Feng H, Wang M, Li Y, Liu Y. Neural Plast. 2017;2017:6809745. doi: 10.1155/2017/6809745. Epub 2017 Oct 22. Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral Ischemia
Gao Y, Li F, Zhang Y, Dai L, Jiang H, Liu H, Zhang S, Chen C, Zhang J. Cell Physiol Biochem. 2013;31(2-3):452-61. Doi: 10.1159/000343381. Epub 2013 Mar 15. Silencing Of Adipoq Efficiently Suppresses Preadipocyte Differentiation In Porcine.
Liu F, Mu J, Yuan Z, Wu G, Liu E, Zheng S, Lian Q, Ren K, Xu H. Nutrition. 2012 Apr;28(4):422-5. Doi: 10.1016/J.Nut.2011.08.018. Epub 2011 Dec 20. High Salt Intake Fails To Enhance Plasma Adiponectin In Normotensive Salt-Sensitive Subjects.
Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Adiponectin/ADIPOQ Antibody Picoband™
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue using anti-Adiponectin/ADIPOQ antibody PB9011. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-29
Question
My boss were content with the WB result of your anti-Adiponectin/ADIPOQ antibody. However we have observed positive staining in adipose tissue secreted. using this antibody. Is that expected? Could you tell me where is ADIPOQ supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
From what I have seen in literature, adipose tissue does express ADIPOQ. Generally ADIPOQ expresses in secreted. Regarding which tissues have ADIPOQ expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8619847
Boster Scientific Support
Answered: 2020-02-27
Question
I am looking for using your anti-Adiponectin/ADIPOQ antibody for cellular response to camp studies. Has this antibody been tested with western blotting on mouse lung? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We appreciate your inquiry. This PB9011 anti-Adiponectin/ADIPOQ antibody is validated on mouse lung, tissue lysate, liver tissue, rat liver tissue, testis tissue, spleen tissue, skeletal muscle tissue, cardiac muscle tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-18
Question
We are currently using anti-Adiponectin/ADIPOQ antibody PB9011 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The anti-Adiponectin/ADIPOQ antibody (PB9011) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-09
Question
Will PB9011 anti-Adiponectin/ADIPOQ antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
You can see on the product datasheet, PB9011 anti-Adiponectin/ADIPOQ antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-11
Question
We ordered your anti-Adiponectin/ADIPOQ antibody for IHC on adipose tissue of abdominal region a few months ago. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining adipose tissue of abdominal region as well as adipose tissue in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
I have checked the website and datasheets of our anti-Adiponectin/ADIPOQ antibody and it appears that PB9011 has been tested on mouse in both IHC and WB. Thus PB9011 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-16
Question
Can you help my question with product PB9011, anti-Adiponectin/ADIPOQ antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9011 anti-Adiponectin/ADIPOQ antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-10
Question
Do you have a BSA free version of anti-Adiponectin/ADIPOQ antibody PB9011 available?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Adiponectin/ADIPOQ antibody PB9011 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-27
Question
Would anti-Adiponectin/ADIPOQ antibody PB9011 work for WB with adipose tissue?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
According to the expression profile of adipose tissue, ADIPOQ is highly expressed in adipose tissue. So, it is likely that anti-Adiponectin/ADIPOQ antibody PB9011 will work for WB with adipose tissue.
Boster Scientific Support
Answered: 2019-06-14
Question
I was wanting to use your anti-Adiponectin/ADIPOQ antibody for WB for rat adipose tissue on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat adipose tissue identification?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
You can see on the product datasheet, PB9011 anti-Adiponectin/ADIPOQ antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat adipose tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-16
Question
Is a blocking peptide available for product anti-Adiponectin/ADIPOQ antibody (PB9011)?
Verified Customer
Verified customer
Asked: 2019-05-08
Answer
We do provide the blocking peptide for product anti-Adiponectin/ADIPOQ antibody (PB9011). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-08
Question
I see that the anti-Adiponectin/ADIPOQ antibody PB9011 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-12
Question
We need to test anti-Adiponectin/ADIPOQ antibody PB9011 on rat adipose tissue for research purposes, then I may be interested in using anti-Adiponectin/ADIPOQ antibody PB9011 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
The products we sell, including anti-Adiponectin/ADIPOQ antibody PB9011, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-07
Question
Is this PB9011 anti-Adiponectin/ADIPOQ antibody reactive to the isotypes of ADIPOQ?
A. Thomas
Verified customer
Asked: 2017-11-02
Answer
The immunogen of PB9011 anti-Adiponectin/ADIPOQ antibody is E.coli-derived rat Adiponectin recombinant protein (Position: I19-N244). Rat Adiponectin shares 90% amino acid (aa) sequence identity with mouse Adiponectin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-02
Question
See attached the WB image, lot number and protocol we used for adipose tissue using anti-Adiponectin/ADIPOQ antibody PB9011. Please let me know if you require anything else.
Z. Krishna
Verified customer
Asked: 2013-10-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-04
Question
We have observed staining in rat adipose tissue of abdominal region. Do you have any suggestions? Is anti-Adiponectin/ADIPOQ antibody supposed to stain adipose tissue of abdominal region positively?
J. Mangal
Verified customer
Asked: 2013-03-04
Answer
According to literature adipose tissue of abdominal region does express ADIPOQ. According to Uniprot.org, ADIPOQ is expressed in adipose tissue of abdominal region, adipose tissue, among other tissues. Regarding which tissues have ADIPOQ expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8619847
Boster Scientific Support
Answered: 2013-03-04