Anti-Adiponectin/ADIPOQ Antibody Picoband™

Adiponectin/Acrp30 antibody

Boster Bio Anti-Adiponectin/ADIPOQ Antibody Picoband™ catalog # PB9011. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: PB9011
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Adiponectin/ADIPOQ Antibody Picoband™

View all Adiponectin/Acrp30 Antibodies

SKU/Catalog Number

PB9011

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Adiponectin/ADIPOQ Antibody Picoband™ catalog # PB9011. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Adiponectin/ADIPOQ Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9011)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived rat Adiponectin recombinant protein (Position: I19-N244). Rat Adiponectin shares 90% amino acid (aa) sequence identity with mouse Adiponectin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9011 is reactive to ADIPOQ in Mouse, Rat

Applications

PB9011 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

26.414kDa

Background of Adiponectin/Acrp30

Adiponectin, also known as APM1 or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. It is mapped to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, it has been showed that Adiponectin is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, it has been found that links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. This gene is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For ADIPOQ (Source: Uniprot.org, NCBI)

Gene Name

ADIPOQ

Full Name

Adiponectin

Weight

26.414kDa

Alternative Names

ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein ADIPOQ ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28 adiponectin, C1Q and collagen domain containing adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement-related 30 kDa protein|adipose most abundant gene transcript 1 protein|adipose specific collagen-like factor|gelatin-binding protein 28

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADIPOQ, check out the ADIPOQ Infographic

ADIPOQ infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADIPOQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9011 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang R, Xie X, Yu Q, Feng H, Wang M, Li Y, Liu Y. Neural Plast. 2017;2017:6809745. doi: 10.1155/2017/6809745. Epub 2017 Oct 22. Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral Ischemia

Gao Y, Li F, Zhang Y, Dai L, Jiang H, Liu H, Zhang S, Chen C, Zhang J. Cell Physiol Biochem. 2013;31(2-3):452-61. Doi: 10.1159/000343381. Epub 2013 Mar 15. Silencing Of Adipoq Efficiently Suppresses Preadipocyte Differentiation In Porcine.

Liu F, Mu J, Yuan Z, Wu G, Liu E, Zheng S, Lian Q, Ren K, Xu H. Nutrition. 2012 Apr;28(4):422-5. Doi: 10.1016/J.Nut.2011.08.018. Epub 2011 Dec 20. High Salt Intake Fails To Enhance Plasma Adiponectin In Normotensive Salt-Sensitive Subjects.

Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...

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16 Customer Q&As for Anti-Adiponectin/ADIPOQ Antibody Picoband™

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue using anti-Adiponectin/ADIPOQ antibody PB9011. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-29

Question

My boss were content with the WB result of your anti-Adiponectin/ADIPOQ antibody. However we have observed positive staining in adipose tissue secreted. using this antibody. Is that expected? Could you tell me where is ADIPOQ supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

From what I have seen in literature, adipose tissue does express ADIPOQ. Generally ADIPOQ expresses in secreted. Regarding which tissues have ADIPOQ expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8619847

Boster Scientific Support

Answered: 2020-02-27

Question

I am looking for using your anti-Adiponectin/ADIPOQ antibody for cellular response to camp studies. Has this antibody been tested with western blotting on mouse lung? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

We appreciate your inquiry. This PB9011 anti-Adiponectin/ADIPOQ antibody is validated on mouse lung, tissue lysate, liver tissue, rat liver tissue, testis tissue, spleen tissue, skeletal muscle tissue, cardiac muscle tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-18

Question

We are currently using anti-Adiponectin/ADIPOQ antibody PB9011 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

The anti-Adiponectin/ADIPOQ antibody (PB9011) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-09

Question

Will PB9011 anti-Adiponectin/ADIPOQ antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

You can see on the product datasheet, PB9011 anti-Adiponectin/ADIPOQ antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-11

Question

We ordered your anti-Adiponectin/ADIPOQ antibody for IHC on adipose tissue of abdominal region a few months ago. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining adipose tissue of abdominal region as well as adipose tissue in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

I have checked the website and datasheets of our anti-Adiponectin/ADIPOQ antibody and it appears that PB9011 has been tested on mouse in both IHC and WB. Thus PB9011 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-16

Question

Can you help my question with product PB9011, anti-Adiponectin/ADIPOQ antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9011 anti-Adiponectin/ADIPOQ antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-10

Question

Do you have a BSA free version of anti-Adiponectin/ADIPOQ antibody PB9011 available?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Adiponectin/ADIPOQ antibody PB9011 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-27

Question

Would anti-Adiponectin/ADIPOQ antibody PB9011 work for WB with adipose tissue?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

According to the expression profile of adipose tissue, ADIPOQ is highly expressed in adipose tissue. So, it is likely that anti-Adiponectin/ADIPOQ antibody PB9011 will work for WB with adipose tissue.

Boster Scientific Support

Answered: 2019-06-14

Question

I was wanting to use your anti-Adiponectin/ADIPOQ antibody for WB for rat adipose tissue on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat adipose tissue identification?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

You can see on the product datasheet, PB9011 anti-Adiponectin/ADIPOQ antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat adipose tissue in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-16

Question

Is a blocking peptide available for product anti-Adiponectin/ADIPOQ antibody (PB9011)?

Verified Customer

Verified customer

Asked: 2019-05-08

Answer

We do provide the blocking peptide for product anti-Adiponectin/ADIPOQ antibody (PB9011). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-08

Question

I see that the anti-Adiponectin/ADIPOQ antibody PB9011 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-03-12

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-03-12

Question

We need to test anti-Adiponectin/ADIPOQ antibody PB9011 on rat adipose tissue for research purposes, then I may be interested in using anti-Adiponectin/ADIPOQ antibody PB9011 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-05-07

Answer

The products we sell, including anti-Adiponectin/ADIPOQ antibody PB9011, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-05-07

Question

Is this PB9011 anti-Adiponectin/ADIPOQ antibody reactive to the isotypes of ADIPOQ?

A. Thomas

Verified customer

Asked: 2017-11-02

Answer

The immunogen of PB9011 anti-Adiponectin/ADIPOQ antibody is E.coli-derived rat Adiponectin recombinant protein (Position: I19-N244). Rat Adiponectin shares 90% amino acid (aa) sequence identity with mouse Adiponectin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-02

Question

See attached the WB image, lot number and protocol we used for adipose tissue using anti-Adiponectin/ADIPOQ antibody PB9011. Please let me know if you require anything else.

Z. Krishna

Verified customer

Asked: 2013-10-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-10-04

Question

We have observed staining in rat adipose tissue of abdominal region. Do you have any suggestions? Is anti-Adiponectin/ADIPOQ antibody supposed to stain adipose tissue of abdominal region positively?

J. Mangal

Verified customer

Asked: 2013-03-04

Answer

According to literature adipose tissue of abdominal region does express ADIPOQ. According to Uniprot.org, ADIPOQ is expressed in adipose tissue of abdominal region, adipose tissue, among other tissues. Regarding which tissues have ADIPOQ expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 8619847

Boster Scientific Support

Answered: 2013-03-04

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Conjugation

Total: $370

SKU:PB9011

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