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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and urine|
|Assay Time:||90 Minutes|
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Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Mouse Adiponectin PicoKine™ Quick ELISA Kit
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse Adiponectin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse Adiponectin PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0596)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E18-N244
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
FEK0596 is reactive to Adipoq in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine
FEK0596 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|FEK0596-CAP||Anti-tag Pre-coated 96-well Strip Microplate||1||12 strips of 8 wells|
|FEK0596-ST||Mouse Adiponectin/Acrp30 Standard||2||20 ng/tube|
|FEK0596-C||Mouse Adipoq Antibody Cocktail||1||6ml|
|AR1106-7||TBS-T Wash Buffer (25x)||1||12ml|
|AR1104||Color Developing Reagent (TMB)||1||10ml|
|PLA-SEA||Adhesive Plate Sealers||2||Piece|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse Adiponectin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Adiponectin/Acrp30 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Adipoq (Source: Uniprot.Org, NCBI)
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein Adipoq|30kDa, APN, Acd, Acdc, Acr, Acrp30, Ad, Adid, GBP2, GBP28, a, adip, adipo, ap, apM1|adiponectin, C1Q and collagen domain containing|adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement related protein|adipocyte complement-related 30 kDa protein|adipocyte, C1Q and collagen domain containing|adipocyte, C1q and collagen domain-containing protein|adipocyte-specific protein AdipoQ|adiponectin d*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Adipoq, check out the Adipoq Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Adipoq: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
FEK0596 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Oral Administration of Resveratrol Alleviates Osteoarthritis Pathology in C57BL/6J Mice Model Induced by a High-Fat Diet
Puerarin Protects Pancreatic ?-Cells in Obese Diabetic Mice via Activation of GLP-1R Signaling
Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway
Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine
Chen, M., Wang, X., Hu, Z., Zhou, H., Xu, Y., Qiu, L.,…, & Ying, Z. (2017). Programming of mouse obesity by maternal exposure to concentrated ambient fine particles. Particle and Fibre Toxicology, 14(1), 20. doi: 10.1186/s12989-017-0201-9
Liu Xx, Liu Ky, Li P, Han S, Peng Xd, Shen L. Diabetes Metab. 2014 Nov;40(5):363-72. Doi: 10.1016/J.Diabet.2014.05.004. Epub 2014 Jun 27. Adiponectin Is Expressed In The Pancreas Of High-Fat-Diet-Fed Mice And Protects Pancreatic Endothelial Functi...
Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni...
Wu Cc, Weng Wl, Lai Wl, Tsai Hp, Liu Wh, Lee Mh, Tsai Yc. Evid Based Complement Alternat Med. 2015;2015:391767. Doi: 10.1155/2015/391767. Epub 2015 Feb 23. Effect Of Lactobacillus Plantarum Strain K21 On High-Fat Diet-Fed Obese Mice.
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