Mouse Adiponectin PicoKine™ Quick ELISA Kit

Mouse Adiponectin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. Cited in 8 publication(s).

Product Info Summary

SKU: FEK0596
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Assay Time: 90 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Mouse Adiponectin PicoKine™ Quick ELISA Kit

See all Adiponectin/Acrp30 products

SKU/Catalog Number

FEK0596

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Adiponectin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Adiponectin PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0596)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-N244

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0596 is reactive to Adipoq in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Application Guarantee

FEK0596 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK0596-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0596-STMouse Adiponectin/Acrp30 Standard 2 20 ng/tube
FEK0596-CMouse Adipoq Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0210.0680.1260.3930.6231.0391.4522.063

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5672994757251231217518
Standard deviation37.98179.64318.0237.75240.31330.79
CV (%)6.7%6%4.2%7.1%7.7%4.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Adiponectin/Acrp30 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 156760362857159224.914.2%
Sample 22994286728452816288067.972.3%
Sample 375727510816774137665295.033.8%
*number of samples for each test n=16.

Gene/Protein Information For Adipoq (Source: Uniprot.Org, NCBI)

Uniprot ID

Q60994

Gene ID

11450

Gene Name

Adipoq

Full Name

Adiponectin

Weight

26.809kDa

Alternative Names

ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein Adipoq|30kDa, APN, Acd, Acdc, Acr, Acrp30, Ad, Adid, GBP2, GBP28, a, adip, adipo, ap, apM1|adiponectin, C1Q and collagen domain containing|adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement related protein|adipocyte complement-related 30 kDa protein|adipocyte, C1Q and collagen domain containing|adipocyte, C1q and collagen domain-containing protein|adipocyte-specific protein AdipoQ|adiponectin d

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Adipoq, check out the Adipoq Infographic

Adipoq infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Adipoq: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

FEK0596 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Oral Administration of Resveratrol Alleviates Osteoarthritis Pathology in C57BL/6J Mice Model Induced by a High-Fat Diet

Puerarin Protects Pancreatic ?-Cells in Obese Diabetic Mice via Activation of GLP-1R Signaling

Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway

Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine

Chen, M., Wang, X., Hu, Z., Zhou, H., Xu, Y., Qiu, L.,…, & Ying, Z. (2017). Programming of mouse obesity by maternal exposure to concentrated ambient fine particles. Particle and Fibre Toxicology, 14(1), 20. doi: 10.1186/s12989-017-0201-9

Liu Xx, Liu Ky, Li P, Han S, Peng Xd, Shen L. Diabetes Metab. 2014 Nov;40(5):363-72. Doi: 10.1016/J.Diabet.2014.05.004. Epub 2014 Jun 27. Adiponectin Is Expressed In The Pancreas Of High-Fat-Diet-Fed Mice And Protects Pancreatic Endothelial Functi...

Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni...

Wu Cc, Weng Wl, Lai Wl, Tsai Hp, Liu Wh, Lee Mh, Tsai Yc. Evid Based Complement Alternat Med. 2015;2015:391767. Doi: 10.1155/2015/391767. Epub 2015 Feb 23. Effect Of Lactobacillus Plantarum Strain K21 On High-Fat Diet-Fed Obese Mice.

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