Anti-Emerin EMD Antibody

Product Name

Anti-Emerin EMD Antibody

View all EMD Antibodies

SKU/Catalog Number

A00714-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Emerin EMD Antibody (Catalog # A00714-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Emerin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Emerin EMD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00714-1)

Host

Rabbit

Contents

Emerin Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Emerin antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Emerin. The immunogen is located within the first 50 amino acids of Emerin.

Reactive Species

A00714-1 is reactive to EMD in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

29.0 kDa

Background of EMD

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family that includes proteins such as LAP2 and MAN1. Each family member, including Emerin, has an ~40 amino acid LEM-domains that binds barrier-to-autointegration (BANF1), a conserved chromatin protein that also serves as a host cell component of retroviral integration complexes, including that of HIV. Emerin is anchored at the inner membrane of the nuclear envelope where it binds to nuclear intermediate filaments that are formed by lamin proteins. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00714-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Emerin antibody can be used for detection of Emerin by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information EMD

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