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Product Info Summary
| SKU: | A08768 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-ENASE Antibody (Center)
SKU/Catalog Number
A08768
Size
80 µl
Description
Boster Bio Anti-ENASE Antibody (Center) (Catalog # A08768). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ENASE Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08768)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08768 is reactive to ENGASE in Human
Reconstitution
Calculated molecular weight
83987 Da
Background of ENGASE
Endo-beta-N-acetylglucosaminidase (ENGase; EC 3.2.1.96) is involved in the processing of free oligosaccharides in the cytosol.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08768 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: human brain tissue lysate
Lane 3: mouse thymus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 84 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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ENASE Antibody (Center) (Cat. #A08768) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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ENASE Antibody (Center) (Cat. #A08768) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For ENGASE (Source: Uniprot.org, NCBI)
Gene Name
ENGASE
Full Name
Cytosolic endo-beta-N-acetylglucosaminidase
Weight
83987 Da
Superfamily
glycosyl hydrolase 85 family
Alternative Names
Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE ENGASE endo-beta-N-acetylglucosaminidase cytosolic endo-beta-N-acetylglucosaminidase|Di-N-acetylchitobiosyl beta-N-acetylglucosaminidase|Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ENGASE, check out the ENGASE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENGASE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ENASE Antibody (Center) (A08768)
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