Product Info Summary
SKU: | M01860 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, WB |
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Product info
Product Name
Anti-EphA3 EPHA4 Monoclonal Antibody
SKU/Catalog Number
M01860
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EphA3 EPHA4 Monoclonal Antibody catalog # M01860. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EphA3 EPHA4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01860)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
6C1B6
Isotype
IgG
Immunogen
Purified recombinant fragment of EphA3 (aa751-983) expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01860 is reactive to EPHA4 in Human
Applications
M01860 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
109.86kDa
Background of EPHA4
Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-EphA3 EPHA4 Monoclonal Antibody M01860
Western Blot (WB) analysis using EphA3 Monoclonal Antibody against truncated Trx-EphA3 recombinant protein (1) and truncated EphA3(aa566-983)-hIgGFc transfected CHO-K1 cell lysate(2).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EPHA4 (Source: Uniprot.org, NCBI)
Gene Name
EPHA4
Full Name
Ephrin type-A receptor 4
Weight
109.86kDa
Superfamily
protein kinase superfamily
Alternative Names
Cek8; EC 2.7.10; EC 2.7.10.1; EK8; EPH receptor A4; EphA4; EPH-like kinase 8; ephrin type-A receptor 4; Hek8; receptor protein-tyrosine kinase HEK8; SEK; Sek1; TYRO1 protein tyrosine kinase; Tyro1; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase TYRO1 EPHA4 EK8, HEK8, SEK, TYRO1 EPH receptor A4 ephrin type-A receptor 4|EPH-like kinase 8|TYRO1 protein tyrosine kinase|receptor protein-tyrosine kinase HEK8|tyrosine-protein kinase TYRO1|tyrosine-protein kinase receptor SEK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHA4, check out the EPHA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EphA3 EPHA4 Monoclonal Antibody (M01860)
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No publications found for M01860
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Recommended Resources
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