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Product Info Summary
| SKU: | A02767 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Ephrin-B1/2 EFNB1 Antibody
SKU/Catalog Number
A02767
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Ephrin-B1/2 EFNB1 Antibody catalog # A02767. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ephrin-B1/2 EFNB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02767)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
8C4C4
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human EFNB1/2. AA range:284-333
Reactive Species
A02767 is reactive to EFNB1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02767 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of 293 cells using Ephrin-B1/2 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 5', using EFNB1/2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human testis tissue, using EFNB1/2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Ephrin-B1/2 EFNB1 Antibody (A02767)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Ephrin-B1/2 EFNB1 Antibody
Question
Would anti-Ephrin-B1/2 antibody A02767 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
According to the expression profile of cervix carcinoma erythroleukemia, EFNB1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Ephrin-B1/2 antibody A02767 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2020-03-02
Question
Do you have a BSA free version of anti-Ephrin-B1/2 antibody A02767 available?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ephrin-B1/2 antibody A02767 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-15
Question
I see that the anti-Ephrin-B1/2 antibody A02767 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-11-21
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Ephrin-B1/2 antibody A02767. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-22
Question
We are currently using anti-Ephrin-B1/2 antibody A02767 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
J. Wu
Verified customer
Asked: 2017-05-08
Answer
The anti-Ephrin-B1/2 antibody (A02767) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-08
