Anti-Ephrin-B1/2 (Q324) EFNB1 Antibody

Product Name

Anti-Ephrin-B1/2 (Q324) EFNB1 Antibody

View all EFNB1 Antibodies

SKU/Catalog Number

A02767-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Ephrin-B1/2 (Q324) EFNB1 Antibody catalog # A02767-1. Tested in IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ephrin-B1/2 (Q324) EFNB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02767-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human TGFbeta RIII around the phosphorylation site of T842.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02767-1 is reactive to EFNB1 in Human, Mouse, Rat

Applications

A02767-1 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

38.007kDa

Background of EFNB1

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Cheung P.C.F., Biochem. J. 346:659-669(2000).
Lang T.M., Genomics 70:258-263(2000).
Ota T., Nat. Genet. 36:40-45(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Immunohistochemistry validation of EFNB1 using Anti-Ephrin-B1/2 (Q324) EFNB1 Antibody (A02767-1).

Immunohistochemistry (IHC) analyzes of Ephrin-B1/2 (Q324) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody

Gene/Protein Information For EFNB1 (Source: Uniprot.org, NCBI)

Alternative Names

Cek5-L; EFL3; EFL-3; EFNB1; ELK ligand; ELK-L; EphrinB1; Ephrin-B1; LERK-2; STRA-1 EFNB1 CFND, CFNS, EFB1, EFL3, EPLG2, Elk-L, LERK2 ephrin B1 ephrin-B1|ELK ligand|eph-related receptor tyrosine kinase ligand 2

For more info on EFNB1, check out the EFNB1 Infographic

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In this infographic, you will see the following information for EFNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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