Anti-ERK1/2 MAPK3 Rabbit Monoclonal Antibody

MAPK3 antibody

Boster Bio Anti-ERK1/2 MAPK3 Rabbit Monoclonal Antibody catalog # M00104-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M00104-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-ERK1/2 MAPK3 Rabbit Monoclonal Antibody

View all MAPK3 Antibodies

SKU/Catalog Number

M00104-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ERK1/2 MAPK3 Rabbit Monoclonal Antibody catalog # M00104-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ERK1/2 MAPK3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00104-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DFF-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ERK1/2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00104-1 is reactive to MAPK3 in Human, Mouse, Rat

Applications

M00104-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

31 kDa, 28 kDa, 17 kDa

Calculated molecular weight

43.136kDa

Background of MAPK3

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:200

Validation Images & Assay Conditions

Gene/Protein Information For MAPK3 (Source: Uniprot.org, NCBI)

Gene Name

MAPK3

Full Name

Mitogen-activated protein kinase 3

Weight

43.136kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; ERK1; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 2.7.11.24 MAPK3 ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK mitogen-activated protein kinase 3 mitogen-activated protein kinase 3|MAPK 1|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1|insulin-stimulated MAP2 kinase|microtubule-associated protein 2 kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAPK3, check out the MAPK3 Infographic

MAPK3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAPK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00104-1 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

CXCL13 inhibition induce the apoptosis of MDA-MB-231 breast cancer cells through blocking CXCR5/ERK signaling pathway.

Huanyue Cui,Xueying Liu,Jin Zhang,Ke Zhang,Dahong Yao,Shi Dong,Shushu Feng,Lu Yang, Yuyao Li,Hangyu Wang,Jian Huang,Jinhui Wang,"Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways",Evidence-Based Complementary and Alternative Medicine,vol. 2021,Article ID 6103158,18 pages,2021.https://doi.org/10.1155/2021/6103158
Species: Human,Rat
M00104-1 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:200

Feng,S.,Wang,S.,Sun,S.,Su,H.,& Zhang,L.(2021).Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats.Molecular Medicine Reports,23,294.h
Species: Rat
M00104-1 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:200

Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14
Species: Mouse
M00104-1 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA

Lin S,Li Q,Jiang S,Xu Z,Jiang Y,Liu L,Jiang J,Tong Y,Wang P.Crocetin ameliorates chronic restraint stress-induced depression-like behaviors in mice by regulating MEK/ERK pathways and gut microbiota. J Ethnopharmacol.2020 Nov 23:113608.doi:10.1016/j.jep.20
Species: Mouse
M00104-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:50

Inhibition of extracellular signal-regulated kinases ameliorates hypertension-induced renal vascular remodeling in rat models

Synthesis and biological evaluation of novel N, N%u2032-disubstituted urea and thiourea derivatives as potential anti-melanoma agents

Disulfiram inhibits TGF-?-induced epithelial-mesenchymal transition and stem-like features in breast cancer via ERK/NF-?B/Snail pathway

Fructus phyllanthi tannin fraction induces apoptosis and inhibits migration and invasion of human lung squamous carcinoma cells in vitro via MAPK/MMP pathways

Shan, M., Qin, J., Jin, F., Han, X., Guan, H., Li, X.,…, & Wang, Y. (2017). Autophagy suppresses isoprenaline-induced M2 macrophage polarization via the ROS/ERK and mTOR signaling pathway. Free Radical Biology and Medicine. Advance online publicat...

Have you used Anti-ERK1/2 MAPK3 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-ERK1/2 MAPK3 Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-ERK1/2 MAPK3 Rabbit Monoclonal Antibody

Question

Would M00104-1 anti-ERK1/2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

You can see on the product datasheet, M00104-1 anti-ERK1/2 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-06

Question

We ordered your anti-ERK1/2 Rabbit Monoclonal antibody for Flow Cytometry on right frontal lobe in the past. I am using human, and We want to use the antibody for IP next. I am interested in examining right frontal lobe as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IP?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

I viewed the website and datasheets of our anti-ERK1/2 Rabbit Monoclonal antibody and it seems that M00104-1 has been validated on human in both Flow Cytometry and IP. Thus M00104-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-14

Question

I am looking for using your anti-ERK1/2 Rabbit Monoclonal antibody for rsk activation studies. Has this antibody been tested with western blotting on hepg2 whole cell lysates? We would like to see some validation images before ordering.

G. Jackson

Verified customer

Asked: 2019-08-01

Answer

We appreciate your inquiry. This M00104-1 anti-ERK1/2 Rabbit Monoclonal antibody is validated on hepg2 whole cell lysates, hela cells. It is guaranteed to work for Flow Cytometry, IP, IF, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-01

Question

I was wanting to use your anti-ERK1/2 Rabbit Monoclonal antibody for IP for mouse lymph on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lymph identification?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

You can see on the product datasheet, M00104-1 anti-ERK1/2 Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lymph in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-12

Question

See attached the WB image, lot number and protocol we used for lymph using anti-ERK1/2 Rabbit Monoclonal antibody M00104-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-08

Question

We have observed staining in human right frontal lobe. Any tips? Is anti-ERK1/2 Rabbit Monoclonal antibody supposed to stain right frontal lobe positively?

Verified Customer

Verified customer

Asked: 2018-12-04

Answer

According to literature right frontal lobe does express MAPK3. According to Uniprot.org, MAPK3 is expressed in right frontal lobe, hepatoma, lymph, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MAPK3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hepatoma, Pubmed ID: 1540184, 7687743
Leukemic T-cell, Pubmed ID: 19690332
Lymph, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-12-04

Question

We need to test anti-ERK1/2 Rabbit Monoclonal antibody M00104-1 on mouse lymph for research purposes, then I may be interested in using anti-ERK1/2 Rabbit Monoclonal antibody M00104-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Anderson

Verified customer

Asked: 2018-11-20

Answer

The products we sell, including anti-ERK1/2 Rabbit Monoclonal antibody M00104-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-11-20

Question

Is this M00104-1 anti-ERK1/2 Rabbit Monoclonal antibody reactive to the isotypes of MAPK3?

Verified Customer

Verified customer

Asked: 2018-08-14

Answer

The immunogen of M00104-1 anti-ERK1/2 Rabbit Monoclonal antibody is A synthesized peptide derived from human ERK1/2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-08-14

Question

Is a blocking peptide available for product anti-ERK1/2 Rabbit Monoclonal antibody (M00104-1)?

E. Williams

Verified customer

Asked: 2018-01-04

Answer

We do provide the blocking peptide for product anti-ERK1/2 Rabbit Monoclonal antibody (M00104-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-01-04

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-ERK1/2 Rabbit Monoclonal antibody M00104-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-12-04

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-12-04

Question

My colleagues were satisfied with the WB result of your anti-ERK1/2 Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma cytoplasm. nucleus. membrane using this antibody. Is that expected? Could you tell me where is MAPK3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-10-16

Answer

Based on literature, cervix carcinoma does express MAPK3. Generally MAPK3 expresses in cytoplasm. nucleus. membrane, caveola. Regarding which tissues have MAPK3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hepatoma, Pubmed ID: 1540184, 7687743
Leukemic T-cell, Pubmed ID: 19690332
Lymph, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-10-16

Question

Is there a BSA free version of anti-ERK1/2 Rabbit Monoclonal antibody M00104-1 available?

Verified Customer

Verified customer

Asked: 2017-09-18

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ERK1/2 Rabbit Monoclonal antibody M00104-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-09-18

Question

Would anti-ERK1/2 Rabbit Monoclonal antibody M00104-1 work for IP with lymph?

Verified Customer

Verified customer

Asked: 2017-08-17

Answer

According to the expression profile of lymph, MAPK3 is highly expressed in lymph. So, it is likely that anti-ERK1/2 Rabbit Monoclonal antibody M00104-1 will work for IP with lymph.

Boster Scientific Support

Answered: 2017-08-17

Question

I see that the anti-ERK1/2 Rabbit Monoclonal antibody M00104-1 works with IP, what is the protocol used to produce the result images on the product page?

J. Jackson

Verified customer

Asked: 2016-12-12

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-12-12

Question

My question regarding product M00104-1, anti-ERK1/2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Taylor

Verified customer

Asked: 2016-01-28

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00104-1 anti-ERK1/2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-01-28

Question

We are currently using anti-ERK1/2 Rabbit Monoclonal antibody M00104-1 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

P. Jackson

Verified customer

Asked: 2014-09-09

Answer

The anti-ERK1/2 Rabbit Monoclonal antibody (M00104-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-09-09

Order DetailsPrice
M00104-1

100μL

$370
M00104-1-30ul

30μL sample

$99
M00104-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M00104-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.