Anti-ERK1/2 Rabbit Monoclonal Antibody

Western blot analysis of ERK1/2 Antibody expression in HepG2 whole cell lysates.

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

ERK/NF-κB signaling is involved in Kv1.3-related M1 macrophages. A Respective western blotting image and densitometry analysis of ERK/NF-κB signaling after transfection with LV-Kv1.3 in THP-1 cells. Data were expressed as mean ± SD (n = 4). B The phosphorylation signal data are depicted in the form of stacked histogram overlaid plots. The mean fluorescence intensity (MFI) of the ERK1/2 and NF-κB p65 phosphorylation signal was expressed as the mean ± SD (n = 3). C Effects of MgTx, PD98059, and Licochalcone B on Kv1.3 and ERK/NF-κB signaling in LV-Kv1.3-transduced THP-1 cells were analyzed by western blotting. Data were expressed as mean ± SD (n = 3). D Respective western blotting image and densitometry analysis of ERK/NF-κB signaling in HK-2 cells co-cultured with the supernatant from LV-Kv1.3-transduced THP-1 cells for 48 h. Data were expressed as mean ± SD (n = 4). E Respective western blotting image and densitometry analysis of ERK/NF-κB signaling in mice subjected to UUO (7 days post-surgery) and IRI. Data were expressed as mean ± SD (n = 4).
Index in PubMed under a CC BY license. PMID: 40324999

Effects of knocking-down ADRB1 CaMKII on mPFC activity and subsequent signals in METH-sired male F1. a Levels of c-Fos protein. b The c-Fos immunostaining. Scale bar, 500 μm /100 μm. c Density of dendritic spine. Scale bar, 50 μm /10 μm. d Levels of β-arestin2 and PKA protein. e Levels of ERK1/2, p-ERK1/2, ERK1/2/p-ERK1/2, CREB, p-CREB, p-CREB/ CREB and ΔFosB protein. F1-METH-Ctrl, METH-sired male F1 mice injected with Ctrl virus. F1-METH-KD, METH-sired male F1 mice injected with KD virus. The data are presented as the Mean ± SD. N.S., P > 0.05. * P < 0.05, ** P < 0.01 vs F1-METH-Ctrl.
Index in PubMed under a CC BY license. PMID: 37857642

Effect of MYH7 R453C mutation on TGF-β/Smad2/3, ERK1/2, NF-κB and PI3K/AKT pathways. (a) The protein expression of TGF-β/Smad2/3 and ERK1/2 cascades were detected by western blotting. (b–d) The protein expression of TGF-β1/2, p-Smad2/3/Smad2/3 and p-ERK1/2/ERK1/2 were quantitated using densitometry. (e) The protein expression of NF-κB signalling was detected by western blotting. (f) Quantitative analysis of the protein expression of p-NF-κB p65 and NF-κB p65. **p < 0.01, ****p < 0.0001. n = 3 biologically independent samples.
Index in PubMed under a CC BY license. PMID: 38862020

Effects of OPN and OVE on activation of MAPK signaling pathways in LPS-stimulated RAW264.7 cells. Expression levels of p-ERK, ERK, p-p38, p-38, p-JNK, and JNK were detected in the same samples for COX-2 detection after 24 h of LPS stimulation. (A) OVE treatment. (B) OPN treatment. All experiments were carried out in triplicates and data are presented as means ± SDs; one-way ANOVA analysis was adopted for multiple comparisons; ###P < 0.001, ####P < 0.0001, compared to the untreated control group; ***P < 0.001 and ****P < 0.0001, compared to the LPS control group.
Index in PubMed under a CC BY license. PMID: 39455284

Immunofluorescent analysis of Hela cells, using ERK1/2 Antibody.

All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.

All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.

IHC analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.