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MAPK3/MAPK1 antibody (M00104-1) performs well in Flow Cytometry, IP, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 56 Publications

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m00104 1 erk12 primary antibodies if testing 12

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IF analysis of ERK1/2 using anti-ERK1/2 antibody (M00104-1).
ERK1/2 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-ERK1/2 Antibody (M00104-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-ERK1/2 Rabbit Monoclonal Antibody

MAPK3/MAPK1 antibody

56 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IP, IF, ICC, WB

Tested Applications

MAPK3/MAPK1 antibody was positively detected by WB in:

human HeLa whole cell, human HepG2 whole cell, human MCF-7 whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse NIH3T3 whole cell, mouse Neuro-2a whole cellICC/IF: Hela cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MAPK3/MAPK1 antibody was positively detected by IHC in:

mouse pancreas, rat kidney, human breast cancer, human colon cancer, human liver cancer, human lung cancer

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

MAPK3/MAPK1 antibody was positively detected by IF in:

mouse pancreas, mouse brain, human prostate cancer, rat brain, rat colon, rat kidney, human breast cancer, human colon cancer, human liver cancer, human pancreatic cancer

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Tested

Recommend

Rat

Tested

Recommend

Tested

Recommend

Mouse

Tested

Recommend

Tested

Recommend

Product Information

Product Name

Anti-ERK1/2 Rabbit Monoclonal Antibody
View all MAPK3/MAPK1 Antibodies

Form

Liquid

Uniprot

P27361/P28482

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

DFF-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ERK1/2

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

41, 43 kDa

Cite This Product

Anti-ERK1/2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00104-1)

Protocols

Western Blot protocol for MAPK3/MAPK1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MAPK3/MAPK1 antibody

Review Protocol

Immunofluorescence (IF) protocol for MAPK3/MAPK1 antibody

Review Protocol

Publications

Cordycepin protects against simulated microgravity–induced osteogenic impairment involving ferroptosis-related signaling

Published: 2026-08-16

Journal: BIOCHEMICAL PHARMACOLOGY

Application: WB

Impact Factor: 6.50

Species: Mouse

PMID: 42603620

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Free Secondary Antibody

Anti-ERK1/2 Rabbit Monoclonal Antibody

MAPK3/MAPK1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Cordycepin protects against simulated microgravity–induced osteogenic impairment involving ferroptosis-related signaling

Exploring the Role of Nesprin1 in isoproterenol-induced heart failure: Insights into pathogenesis and therapeutic implications

Development of Oncolytic Virus-Proteolysis-Targeting Chimera Conjugates for Tumor Immunotherapy

Licochalcone a Disrupted Mitochondrial Function to Promote Cuproptosis in Glioblastoma Through Regulating RAS/MAPK Pathway

TRPM3 on Glutamatergic Neurons in Anterior Cingulate Cortex Modulates Retrieval of Methamphetamine Reward-Associated Context Memory

Anti-asthmatic effects and ingredient annotation of the active fraction from Ganoderma lucidum

A novel polysaccharide from Polygonatum cyrtonema fibrous root mediates anti-diarrheal effects by reprogramming macrophage M1/M2 polarization

In vivo anti-inflammatory, analgesic activities and phytochemical profile of Hu Lisan ethanol extract

Farnesyl Pyrophosphate Synthase Promotes Restenosis After Vascular Injury by Activating Small G Proteins

miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway

Therapeutic potential of voltage-dependent potassium channel subtype 1.3 blockade in alleviating macrophage-related renal inflammation and fibrogenesis

Citrus medica L. essential oil improves anxiety-like behaviors accompanied by fatigue through α7-nAChR receptor-mediated neuroprotection effects

Anti-inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF-κB and MAPK signaling pathways

Ursolic acid, an inhibitor of TMEM16A, co-loaded with cisplatin in hydrogel drug delivery system for multi-targeted therapy of lung cancer

MYH7 R453C induced cardiac remodelling via activating TGF-β/Smad2/3, ERK1/2 and Nox4/ROS/NF-κB signalling pathways

Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study

Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway

Rosavin Alleviates LPS-Induced Acute Lung Injure by Modulating the TLR-4/NF-κB/MAPK Singnaling Pathways

The mechanism of blood coagulation induced by sodium dehydroacetate via the regulation of the mTOR/ERK pathway in rats

Accelerated osteogenesis of bone graft by optimizing the bone microenvironment formed by electrical signals dependent on driving micro vibration stimulation

Involvement of PI3K/AKT Pathway in the Rapid Antidepressant Effects of Crocetin in Mice with Depression-Like Phenotypes

Paternal methamphetamine exposure induces higher sensitivity to methamphetamine in male offspring through driving ADRB1 on CaMKII-positive neurons in mPFC

Transcriptome, network analysis, and molecular docking approaches to elucidate the modes of action of Astragaloside IV against myocardial infarction with mitochondrial energy metabolism network-targeted regulation

Structural characteristics and immunomodulation activity of a polysaccharide from purslane (Portulaca oleracea)

mPFC DUSP1 mediates adolescent cocaine exposure-induced higher sensitivity to drug in adulthood

Identification of several inflammation-related genes based on bioinformatics and experiments

Dopamine receptor 1 on CaMKII-positive neurons within claustrum mediates adolescent cocaine exposure-induced anxiety-like behaviors and electro-acupuncture therapy

Patchouli alcohol against renal fibrosis of spontaneously hypertensive rats via Ras/Raf-1/ERK1/2 signalling pathway

RNF113A targeted by miR-197 promotes proliferation and inhibits autophagy via CXCR4/CXCL12/AKT/ERK/Beclin1 axis in cervical cancer

Dioscin induces osteosarcoma cell apoptosis by upregulating ROS-mediated P38 MAPK signaling

Ameliorative effects of epigallocatechin-3-gallate nanoparticles on 2,4-dinitrochlorobenzene induced atopic dermatitis: A potential mechanism of inflammation-related necroptosis

Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer

Fumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor

Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways

Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats

Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulating matrix metalloproteinase 1 expression through inhibition of the ERK1/2/NF-κB signalling pathway

Tetrandrine attenuates hyperoxia-induced lung injury in newborn rats via NF-κB p65 and ERK1/2 pathway inhibition

Iridoid glycosides from Morinda officinalis How. exert anti-inflammatory and anti-arthritic effects through inactivating MAPK and NF-κB signaling pathways

Endosulfan promotes cell migration via PTP4A3-mediated signaling pathways in HUVECs

Morin alleviates LPS-induced mastitis by inhibiting the PI3K/AKT, MAPK, NF-κB and NLRP3 signaling pathway and protecting the integrity of blood-milk barrier

Grape Seed Procyanidins Attenuates Cisplatin-induced Human Embryonic Renal Cell Cytotoxicity by Modulating Heme Oxygenase-1 in Vitro

Medical findings of nasopharyngeal carcinoma patients and anti-tumor benefits of formononetin

Reciprocal stimulating effects of bFGF and FSH on chicken primordial follicle activation through AKT and ERK pathway

Polyamine analogue QMA attenuated ischemic injury in MCAO rats via ERK and Akt activated Nrf2/HO-1 signaling pathway

Association of astragaloside IV-inhibited autophagy and mineralization in vascular smooth muscle cells with lncRNA H19 and DUSP5-mediated ERK signaling

CXCL13 inhibition induce the apoptosis of MDA-MB-231 breast cancer cells through blocking CXCR5/ERK signaling pathway

Protective effects of extracts of Schisandra chinensis stems against acetaminophen-induced hepatotoxicity via regulation of MAPK and caspase-3 signaling pathways

A cardiac glycoside HTF-1 isolated from Helleborus thibetanus Franch displays potent in vitro anti-cancer activity via caspase-9, MAPK and PI3K-Akt-mTOR pathways

Protection of insulin‑like growth factor 1 on experimental peripheral neuropathy in diabetic mice

Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress

TMEM88 mediates inflammatory cytokines secretion by regulating JNK/P38 and canonical Wnt/β-catenin signaling pathway in LX-2 cells

Buyang Huanwu Decoction Ameliorates Poststroke Depression via Promoting Neurotrophic Pathway Mediated Neuroprotection and Neurogenesis

Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma

Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line

Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells

Panax Notoginseng Saponins Promote Osteogenic Differentiation of Bone Marrow Stromal Cells Through the ERK and P38 MAPK Signaling Pathways

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