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Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Antibody (Monoclonal, MAPK-YT)

SKU MA1055
Size 100μg/vial
Reactivity Human, Mouse, Rat, Yeast
Clonality Monoclonal
Clone Number MAPK-YT
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, ICC, WB

Overview

Product Name Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Antibody (Monoclonal, MAPK-YT)
SKU/Catalog Number MA1055
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for MAP Kinase, Activated (Diphosphorylated ERK-1&2), mitogen-activated protein kinase 1; mitogen-activated protein kinase 3 (MAPK1; MAPK3 ) detection. Tested with WB, IHC-P, ICC in Human;mouse;rat;yeast. No cross reactivity with other proteins.
Cite This Product Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Antibody (Monoclonal, MAPK-YT) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1055)
Host Mouse
Immunogen A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.
Reactivity Human, Mouse, Rat, Yeast

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat, yeast, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, yeast, -
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, yeast

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P21708
Gene Name Mapk3
Protein Name Mitogen-activated protein kinase 3
Tissue Specificity Highest levels within the nervous system, expressed in different tissues, mostly in intestine, placenta and lung.
Alternative Names Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;2.7.11.24;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;MNK1;Microtubule-associated protein 2 kinase;p44-ERK1;Mapk3;Erk1, Prkm3;
Subcellular Localization Cytoplasm . Nucleus. Autophosphorylation at Thr-207 promotes nuclear localization. PEA15-binding redirects the biological outcome of MAPK3 kinase- signaling by sequestering MAPK3 into the cytoplasm. .
Molecular Weight 43081 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Background In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein(MAP) kinase pathways. The central core of each MAP kinase(MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase(MAPKKK) phosphorylates and activates a specific MAPK kinase(MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.

Order Product (MA1055)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

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Publications

Li Xl, Chen Xq, Zhang Mn, Chen N, Nie L, Xu M, Gong J, Shen Pf, Su Zz, Weng X, Tan Jy, Zhao T, Zeng H, Zhou Q. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3871-81. Ecollection 2015. Sox9 Was Involved In Tkis Resistance In Renal Cell Carcinoma Via Raf/M...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.