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Product Info Summary
|Reactive Species:||Human, Mouse, Rat, Yeast|
|Application:||IHC, ICC, WB|
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Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT)
Boster Bio Anti-Phospho-MAP Kinase, Activated (Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) catalog # MA1055. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Yeast.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1055)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
MA1055 is reactive to Mapk3 in Human, Mouse, Rat, Yeast
MA1055 is guaranteed for IHC, ICC, WB Boster Guarantee
Background of Mapk3
In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein (MAP) kinase pathways. The central core of each MAP kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat, yeast, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, yeast, -
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, yeast
Validation Images & Assay Conditions
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Boster Kit Box
Gene/Protein Information For Mapk3 (Source: Uniprot.Org, NCBI)
Mitogen-activated protein kinase 3
protein kinase superfamily
EC 2.7.11; ERK1; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 184.108.40.206 Mapk3|ERK1, ERT2, Erk-1, Esrk1, MAPK1, MNK1, Prkm3, p44, p44erk1, p44mapk|mitogen activated protein kinase 3|mitogen-activated protein kinase 3|MAP kinase 1|MAP kinase 3|MAPK 1|MAPK 3|extracellular signal-regulated kinase 1|insulin-stimulated MAP2 kinase|microtubule-associated protein 2 kinase|mitogen-activated 3|mitogen-activated protein kinase 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Mapk3, check out the Mapk3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Mapk3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) (MA1055)
MA1055 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mehrdoost, S., Yaghmaei, P., Jafary, H., Ebrahim-Habibi, A. (2021). The therapeutic effects of berberine plus sitagliptin in a rat model of fatty liver disease. Iranian Journal of Basic Medical Sciences, 24(4), 451-459. doi: 10.22038/ijbms.2021.52239.11822
MA1055 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Huanyue Cui,Xueying Liu,Jin Zhang,Ke Zhang,Dahong Yao,Shi Dong,Shushu Feng,Lu Yang, Yuyao Li,Hangyu Wang,Jian Huang,Jinhui Wang,"Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways",Evidence-Based Complementary and Alternative Medicine,vol. 2021,Article ID 6103158,18 pages,2021.https://doi.org/10.1155/2021/6103158
MA1055 usage in article: APP: WB, SAMPLE:LUNG TISSUE, DILUTION:1:200
Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14
MA1055 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA
Ji F,Wang Y,Yuan J,Wu Q,Wang J,Liu D.The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells.J Cell Physiol.2020 Apr;235(4):3548-3557.doi:10.1002/jcp.29243.Epub 2019 Sep 30.PMID:31566725.
MA1055 usage in article: APP:IF, SAMPLE:ADSCS, DILUTION:1:400
Wang C,Wang J,Liu X,Han Z,Aimin Jiang,Wei Z,Yang Z.Cl-amidine attenuates lipopolysaccharide-induced mouse mastitis by inhibiting NF-κB, MAPK, NLRP3 signaling pathway and neutrophils extracellular traps release.Microb Pathog.2020 Sep 24;149:104530.doi:10.1
Upregulation of long noncoding rna ccaT1-l promotes epithelial%u2013mesenchymal transition in gastric adenocarcinoma
Li Xl, Chen Xq, Zhang Mn, Chen N, Nie L, Xu M, Gong J, Shen Pf, Su Zz, Weng X, Tan Jy, Zhao T, Zeng H, Zhou Q. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3871-81. Ecollection 2015. Sox9 Was Involved In Tkis Resistance In Renal Cell Carcinoma Via Raf/M...
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