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Product Info Summary
| SKU: | MA1055 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat, Yeast |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT)
SKU/Catalog Number
MA1055
BM1595 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Phospho-MAP Kinase, Activated (Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) catalog # MA1055. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Yeast.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1055)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
MAPK-YT
Isotype
Mouse IgG1
Immunogen
A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1055 is reactive to Mapk3 in Human, Mouse, Rat, Yeast
Observed Molecular Weight
42 kDa
Calculated molecular weight
43.1 kDa
Background of Mapk3
In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein (MAP) kinase pathways. The central core of each MAP kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1055 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat, yeast
Immunocytochemistry , 1μg/ml, Human, mouse, rat, yeast, -
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, yeast
Positive Control
WB: human K562 whole cell
Validation Images & Assay Conditions
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Western blot analysis of MAP kinase,activated using anti-MAP kinase,activated antibody (MA1055).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MAP kinase,activated antigen affinity purified monoclonal antibody (Catalog # MA1055) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MAP kinase,activated at approximately 42 kDa. The expected band size for MAP kinase,activated is at 41 kDa.
Specific Publications For Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) (MA1055)
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