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Product Info Summary
| SKU: | A00030 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-MAPK1 Antibody (C-term)
SKU/Catalog Number
A00030
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-MAPK1 Antibody (C-term) (Catalog # A00030). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MAPK1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00030)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-345 amino acids from the C-terminal region of human MAPK1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00030 is reactive to MAPK1 in Human
Calculated molecular weight
41.4 kDa
Background of MAPK1
MAPK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00030 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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MAPK1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane). This demonstrates the MAPK1 antibody detected the MAPK1 protein (arrow).
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAPK1 Antibody (C-term) (Cat. #A00030), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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MAPK1 Antibody (C-term) (Cat. #A00030) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-MAPK1 Antibody (C-term) (A00030)
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