Anti-FH Antibody Picoband™ (monoclonal, 9D8)

Fumarase antibody

Boster Bio Anti-FH Antibody Picoband™ (monoclonal, 9D8) catalog # M02097. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Product Info Summary

SKU: M02097
Size: 100 μg/vial
Reactive Species: Human, Monkey, Mouse, Rat
Host: Mouse
Application: IF, IHC, ICC, WB

Product Name

Anti-FH Antibody Picoband™ (monoclonal, 9D8)

View all Fumarase Antibodies

SKU/Catalog Number

M02097

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FH Antibody Picoband™ (monoclonal, 9D8) catalog # M02097. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FH Antibody Picoband™ (monoclonal, 9D8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02097)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

9D8

Isotype

Mouse IgG2a

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human FH, which shares 100% and 97.8% amino acid (aa) sequence identity with mouse and rat FH, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M02097 is reactive to FH in Human, Monkey, Mouse, Rat

Applications

M02097 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

48 kDa

Calculated molecular weight

54.637kDa

Background of Fumarase

Fumarase (or fumaratehydratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular localization in the cytosolic form is established by the absence of the signal sequence found in the mitochondrial variety. This enzyme participates in 2 metabolic pathways: citric acid cycle, reductive citric acid cycle (CO2 fixation), and is also important in renal cell carcinoma. Mutations in this gene have been associated with the development of leiomyomas in the skin and uterus in combination with renal cell carcinoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5 μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For FH (Source: Uniprot.org, NCBI)

Gene Name

FH

Full Name

Fumarate hydratase, mitochondrial

Weight

54.637kDa

Superfamily

class-II fumarase/aspartase family

Alternative Names

EC 4.2.1.2; fumarase; fumarate hydratase; fumarate hydratase, mitochondrial; HLRCC; LRCC; MCL; MCUL1 FH FMRD, HLRCC, HsFH, LRCC, MCL, MCUL1 fumarate hydratase fumarate hydratase, mitochondrial|epididymis secretory sperm binding protein|fumarase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FH, check out the FH Infographic

FH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-FH Antibody Picoband™ (monoclonal, 9D8)

Question

Does anti-FH antibody (monoclonal, 9D8) M02097 work for IHC with liver?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

According to the expression profile of liver, FH is highly expressed in liver. So, it is likely that anti-FH antibody (monoclonal, 9D8) M02097 will work for IHC with liver.

Boster Scientific Support

Answered: 2019-12-06

Question

We are currently using anti-FH antibody (monoclonal, 9D8) M02097 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2017-09-15

Answer

The anti-FH antibody (monoclonal, 9D8) (M02097) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-15

Question

I was wanting to use your anti-FH antibody (monoclonal, 9D8) for IHC for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?

M. Taylor

Verified customer

Asked: 2013-05-06

Answer

It shows on the product datasheet, M02097 anti-FH antibody (monoclonal, 9D8) has been validated for IHC, WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-05-06

Order DetailsPrice
M02097

100μg

$370
M02097-10ug

10μg sample (liquid)

$99
M02097-Biotin

100 μg Biotin conjugated

$570
M02097-Cy3

100 μg Cy3 conjugated

$570
M02097-Dylight488

100 μg Dylight488 conjugated

$570
M02097-Dylight550

100 μg Dylight550 conjugated

$570
M02097-Dylight594

100 μg Dylight594 conjugated

$570
M02097-FITC

100 μg FITC conjugated

$570
M02097-HRP

100 μg HRP conjugated

$570
M02097-APC

100 μg APC conjugated

$670
M02097-PE

100 μg PE conjugated

$670
M02097-carrier-free

Carrier Free

$370

More conjugates/formats

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M02097
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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