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Product Info Summary
| SKU: | M00589-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-EWSR1 Mouse Monoclonal Antibody [Clone ID: OTI3F6]
SKU/Catalog Number
M00589-1
Size
100 µl
Description
Boster Bio EWSR1 mouse monoclonal antibody,clone OTI3F6. Catalog# M00589-1. Tested in IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EWSR1 Mouse Monoclonal Antibody [Clone ID: OTI3F6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00589-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3F6
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 256-447 of human EWSR1 (NP_005234) produced in E.coli.
Reactive Species
M00589-1 is reactive to EWSR1 in Human
Calculated molecular weight
68.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00589-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500
IHC 1:500
IF 1:100
Validation Images & Assay Conditions
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Immunofluorescent staining of NCI-H1299 cells using anti-EWSR1 antibody (M00589-1/green
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-EWSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-EWSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-EWSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EWSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EWSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-EWSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-EWSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-EWSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-EWSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-EWSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EWSR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EWSR1 (1:500).
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Western blot analysis of extracts (35ug) from 10 different cell lines by using anti-EWSR1 monoclonal antibody (1:500).
Specific Publications For Anti-EWSR1 Mouse Monoclonal Antibody [Clone ID: OTI3F6] (M00589-1)
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