Product Info Summary
SKU: | M01750-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IF, WB |
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Product info
Product Name
Anti-Ezrin (EZR) Mouse Monoclonal Antibody [Clone ID: OTI1F3]
SKU/Catalog Number
M01750-1
Size
100 µl
Description
Boster Bio EZR mouse monoclonal antibody,clone OTI1F3. Catalog# M01750-1. Tested in IF, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ezrin (EZR) Mouse Monoclonal Antibody [Clone ID: OTI1F3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01750-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1F3
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human EZR (NP_003370) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01750-1 is reactive to EZR in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
69.413kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01750-1 is guaranteed for IF, WB Boster Guarantee
Validation Images & Assay Conditions
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Immunofluorescent staining of OVCAR3 cells using anti-EZR mouse monoclonal antibody (M01750-1
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EZR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EZR (1:500).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and EZR-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-EZR monoclonal antibody M01750-1 (1:250). Then the blotted membrane was stripped and reprobed with anti-actin antibody as a loading control.
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Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-EZR monoclonal antibody (1:500).
Protein Target Info & Infographic
Gene/Protein Information For EZR (Source: Uniprot.org, NCBI)
Gene Name
EZR
Full Name
Ezrin
Weight
69.413kDa
Alternative Names
CVIL; CVL; Cytovillin 2; Cytovillin; DKFZp762H157; EZR; Ezrin; FLJ26216; p81; VIL2; VIL2MGC1584; villin 2 (ezrin); Villin 2; villin-2 EZR CVIL, CVL, HEL-S-105, VIL2 ezrin ezrin|cytovillin 2|epididymis secretory protein Li 105|p81|villin 2 (ezrin)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EZR, check out the EZR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EZR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ezrin (EZR) Mouse Monoclonal Antibody [Clone ID: OTI1F3] (M01750-1)
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