Click image to see more details
Product Info Summary
| SKU: | PA1975 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DR4/TNFRSF10A Antibody Picoband®
SKU/Catalog Number
PA1975
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DR4/TNFRSF10A Antibody catalog # PA1975. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DR4/TNFRSF10A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1975)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human DR4.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1975 is reactive to TNFRSF10A in Human
Observed Molecular Weight
150 kDa
Calculated molecular weight
50.1 kDa
Background of TNFRSF10A
TNFRSF10A (Tumor Necrosis Factor Receptor Subfamily Member 10A), also known as APO2, DR4 or TRAILR1, is a protein that in humans is encoded by the TNFRSF10A gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. By analysis of radiation hybrids, Marsters et al. (1997) mapped the DR4 gene to 8p21. The TRAIL receptor DR5, and 2 decoy receptors for TRAIL, DCR1, and DCR2, are located in the same region, suggesting that these receptors arose from recent gene duplication events. Pan et al. (1997) found that, as with FAS, TNFR1, and DR3, overexpression of DR4 induced apoptosis. However, unlike the other 3 death receptors, DR4 did not use FADD to transmit the death signal, suggesting the use of distinct proximal signaling machinery.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1975 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SW620 Cell, COLO320 Cell, HT1080 Cell,
Validation Images & Assay Conditions
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Anti-DR4 antibody, PA1975, Western blotting
Lane 1: SW620 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HT1080 Cell Lysate
Specific Publications For Anti-DR4/TNFRSF10A Antibody Picoband® (PA1975)
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Customer Q&As
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6 Customer Q&As for Anti-DR4/TNFRSF10A Antibody Picoband®
Question
I see that the anti-DR4/TNFRSF10A antibody PA1975 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-01
Question
We are currently using anti-DR4/TNFRSF10A antibody PA1975 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
The anti-DR4/TNFRSF10A antibody (PA1975) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-DR4/TNFRSF10A antibody PA1975. Let me know if you need anything else.
T. Parker
Verified customer
Asked: 2018-04-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-17
Question
Does PA1975 anti-DR4/TNFRSF10A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
You can see on the product datasheet, PA1975 anti-DR4/TNFRSF10A antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-13
Question
I was wanting to use your anti-DR4/TNFRSF10A antibody for WB for human colon carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
It shows on the product datasheet, PA1975 anti-DR4/TNFRSF10A antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-07
Question
Does anti-DR4/TNFRSF10A antibody PA1975 work for WB with colon carcinoma?
M. Jackson
Verified customer
Asked: 2014-11-13
Answer
According to the expression profile of colon carcinoma, TNFRSF10A is highly expressed in colon carcinoma. So, it is likely that anti-DR4/TNFRSF10A antibody PA1975 will work for WB with colon carcinoma.
Boster Scientific Support
Answered: 2014-11-13
