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- Table of Contents
TNFRSF10A antibody (PA1975) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Anti-DR4 antibody, PA1975, Western blotting
Lane 1: SW620 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 1: SW620 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HT1080 Cell Lysate
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Anti-DR4/TNFRSF10A Antibody Picoband®
TNFRSF10A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
TNFRSF10A antibody was positively detected by WB in:
SW620 Cell, COLO320 Cell, HT1080 Cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-DR4/TNFRSF10A Antibody Picoband®
View all TNFRSF10A Antibodies
View all TNFRSF10A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human DR4.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated Molecular Weight
50.1 kDa
Background of TNFRSF10A
TNFRSF10A (Tumor Necrosis Factor Receptor Subfamily Member 10A), also known as APO2, DR4 or TRAILR1, is a protein that in humans is encoded by the TNFRSF10A gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. By analysis of radiation hybrids, Marsters et al. (1997) mapped the DR4 gene to 8p21. The TRAIL receptor DR5, and 2 decoy receptors for TRAIL, DCR1, and DCR2, are located in the same region, suggesting that these receptors arose from recent gene duplication events. Pan et al. (1997) found that, as with FAS, TNFR1, and DR3, overexpression of DR4 induced apoptosis. However, unlike the other 3 death receptors, DR4 did not use FADD to transmit the death signal, suggesting the use of distinct proximal signaling machinery.
Cite This Product
Anti-DR4/TNFRSF10A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1975)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
88%
Moderate Homology
Rat
31%
Low homology
Mouse
25%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TNFRSF10A antibody
Publications
Application:
Zhang L, Niu T, Yang Sy, Lu Z, Chen B. Spine (Phila Pa 1976). 2008 Feb 15;33(4):422-7. Doi: 10.1097/Brs.0B013E318163E036. The Occurrence And Regional Distribution Of Dr4 On Herniated Disc Cells: A Potential Apoptosis Pathway In Lumbar Intervertebr...
PMID: 18277875
Fu K, Ren H, Wang Y, Fei E, Wang H, Wang G. Oncogene. 2012 Mar 8;31(10):1311-22. Doi: 10.1038/Onc.2011.315. Epub 2011 Jul 25. Dj-1 Inhibits Trail-Induced Apoptosis By Blocking Pro-Caspase-8 Recruitment To Fadd.
PMID: 21785459
Liu N, Zuo C, Wang X, Chen T, Yang D, Wang J, Zhu H. Oncotarget. 2014 Jul 15;5(13):4959-71. Mir-942 Decreases Trail-Induced Apoptosis Through Isg12A Downregulation And Is Regulated By Akt.
PMID: 24970806
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Product Promise
We promise Anti-DR4/TNFRSF10A Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1975
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026