This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
RIPK1 antibody (PB9116) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
Filter:
Western blot analysis of RIP using anti-RIP antibody (PB9116).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human RIP protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP antigen affinity purified polyclonal antibody (Catalog # PB9116) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP at approximately 38 kDa. The expected band size for RIP is at 38 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human RIP protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP antigen affinity purified polyclonal antibody (Catalog # PB9116) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP at approximately 38 kDa. The expected band size for RIP is at 38 kDa.
Bundle Save
Anti-RIP/RIPK1 Antibody Picoband®
RIPK1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
RIPK1 antibody was positively detected by WB in:
human Jurkat whole cell, human 22RV1 whole cell, human MCF-7 whole cell, human Hela whole cell, human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-RIP/RIPK1 Antibody Picoband®
View all RIPK1 Antibodies
View all RIPK1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RIP recombinant protein (Position: K316-N671). Human RIP shares 65% amino acid (aa) sequence identity with mouse RIP.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
76 kDa
Calculated Molecular Weight
75.9 kDa
Background of RIPK1
RIPK1, also known as RIP or RIP1, is an enzyme that in humans is encoded by the RIPK1 gene. It is mapped to 6p25.2. RIPK1 is a key signaling molecule in the programmed necrosis pathway, which plays important roles in development, tissue damage response, and antiviral immunity. RIPK1 is known to have function in a variety of cellular pathways including the NF-κB pathway and programmed necrotic cell death (necroptosis). The kinase domain, while important for necroptotic (programmed necrotic) functions, it appears dispensable for other lethal, as well as pro-survival roles. Also, proteolytic processing of RIPk1, through both caspase-dependent and -independent mechanisms, triggers lethality that is dependent on the generation of one or more specific C-terminal cleavage product (s) of RIPk1 upon stress.
Cite This Product
Anti-RIP/RIPK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9116)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
93%
High Homology
Dog
78%
Moderate Homology
Rabbit
75%
Moderate Homology
Cow
74%
Moderate Homology
Sheep
73%
Moderate Homology
Rat
65%
Low homology
Mouse
65%
Low homology
Zebrafish
31%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for RIPK1 antibody
Publications
Application:
Species:
Jujuboside B promotes the death of acute leukemia cell in a RIPK1/RIPK3/MLKL pathway-dependent manner
Published: 2020-03-03
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 3.26
Species: Human
PMID: 32142769
The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice
Published: 2014-12-01
Journal: International Journal of Clinical and Experimental Pathology
Impact Factor: 1.78
PMID: 25674205
Review
Have you used Anti-RIP/RIPK1 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-RIP/RIPK1 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9116
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026