Anti-FAM59A GAREM1 Antibody

Product Name

Anti-FAM59A GAREM1 Antibody

View all FAM59A Antibodies

SKU/Catalog Number

A12630

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FAM59A GAREM1 Antibody (Catalog # A12630). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

FAM59A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-FAM59A GAREM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12630)

Host

Rabbit

Contents

FAM59A Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FAM59A antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAM59A. The immunogen is located within amino acids 630 - 680 of FAM59A.

Reactive Species

A12630 is reactive to FAM59A in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

97.2 kDa

Background of FAM59A

FAM59A, also known as GAREM, was initially identified through a mass spectroscoptic method that measures different phosphotyrosine states of proteins in response to different cell stimuli. FAM59A is a downstream molecule in the EGF signaling pathway that is tyrosine-phosphorylated, and this phosphorylation is needed for the binding of Grb2, an adaptor protein crucial to the transduction of growth signals from the plasma membrane to the nucleus. ERK activation in response to EGF stimulation is regulated by FAM59A in COS-7 and HeLa cells, and the overexpression of FAM59A stimulated cell proliferation and colony formation in soft agar, suggesting that FAM59A might be a critical protein with roles in ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12630 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FAM59A antibody can be used for detection of FAM59A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information FAM59A

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