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Product Info Summary
| SKU: | A08518 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Fat Free VPS51 Antibody
SKU/Catalog Number
A08518
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Fat Free VPS51 Antibody (Catalog # A08518). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Fat Free antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Fat Free VPS51 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08518)
Host
Rabbit
Contents
Fat Free Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free. The immunogen is located within amino acids 620 - 670 of Fat Free.
Cross-reactivity
At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.
Reactive Species
A08518 is reactive to C11ORF2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
86.0 kDa
Background of C11ORF2
Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08518 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Fat Free antibody can be used for detection of Fat Free by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 μg/mL.
Specific Publications For Anti-Fat Free VPS51 Antibody (A08518)
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