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Product Info Summary
| SKU: | A05956-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-FCRL4 Antibody (C-term)
SKU/Catalog Number
A05956-1
Size
80 µl
Description
Boster Bio Anti-FCRL4 Antibody (C-term) (Catalog # A05956-1). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-FCRL4 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05956-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This FCRL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480-507 amino acids from the C-terminal region of human FCRL4.
Reactive Species
A05956-1 is reactive to FCRL4 in Human
Calculated molecular weight
57.2 kDa
Background of FCRL4
This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains three immune-receptor tyrosine-based inhibitory motifs. This protein may play a role in the function of memory B-cells in the epithelia. Aberrations in the chromosomal region encoding this gene are associated with non-Hodgkin lymphoma and multiple myeloma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05956-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-FCRL4 Antibody (C-term) at 1:2000 dilution
Lane 1: Daudi whole cell lysate
Lane 2: Raji whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-FCRL4 Antibody (C-term) at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: Ramos whole cell lysate
Lane 3: Raji whole cell lysate
Lane 4: Daudi whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
FCRL4 Antibody (C-term) (Cat. #A05956-1) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-FCRL4 Antibody (C-term) (A05956-1)
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