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Product Info Summary
| SKU: | A05085 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CD180/Rp105 Antibody
SKU/Catalog Number
A05085
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD180/Rp105 Antibody catalog # A05085. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD180/Rp105 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05085)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from CD180 antigen at AA range: 221-270
Reactive Species
A05085 is reactive to CD180 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
74.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05085 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of mouse brain cells using CD180 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000
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Western Blot analysis of MOUSE-BRAIN cells using CD180 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-CD180/Rp105 Antibody (A05085)
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5 Customer Q&As for Anti-CD180/Rp105 Antibody
Question
Is there a BSA free version of anti-CD180/Rp105 antibody A05085 available?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD180/Rp105 antibody A05085 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-06
Question
I am looking for to test anti-CD180/Rp105 antibody A05085 on mouse b-cell lymphoma for research purposes, then I may be interested in using anti-CD180/Rp105 antibody A05085 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
The products we sell, including anti-CD180/Rp105 antibody A05085, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-26
Question
We are currently using anti-CD180/Rp105 antibody A05085 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
The anti-CD180/Rp105 antibody (A05085) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-10
Question
Will A05085 anti-CD180/Rp105 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Carter
Verified customer
Asked: 2019-05-24
Answer
You can see on the product datasheet, A05085 anti-CD180/Rp105 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-24
Question
I see that the anti-CD180/Rp105 antibody A05085 works with WB, what is the protocol used to produce the result images on the product page?
A. Parker
Verified customer
Asked: 2015-11-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-11-26
