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Product Info Summary
| SKU: | A00802 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Fibroblast growth factor 21 FGF21 Antibody
SKU/Catalog Number
A00802
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Fibroblast growth factor 21 FGF21 Antibody catalog # A00802. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibroblast growth factor 21 FGF21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00802)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
recombinant protein
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00802 is reactive to FGF21 in Human, Mouse, Rat
Calculated molecular weight
22.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00802 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of FGF21 on rat liver cells lysates using anti-FGF21 antibody at 1/1,000 dilution.
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-FGF21 antibody. Counter stained with hematoxylin.
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Effect of TIF1γ treatment on myokine expression in the quadricep muscles of db/db mice. (A) mRNA expression levels of the myokines FGF‐21 , BDNF and FNDC5 were analysed using qPCR. (B) Representative immunoblot images and quantitative analysis of FNDC5 protein expression. (C) Effect of TIF1γ plasmid (40 μg) treatment on serum irisin levels, measured using an ELISA. (D) Changes in serum irisin levels over time in normal male C57BL/6 mice. Statistical significance was determined using a one‐way analysis of variance followed by Tukey's multiple comparison test. Data are presented as mean ± SEM. * p < 0.05. TIF1γ, transcriptional intermediary factor 1γ; qPCR, quantitative polymerase chain reaction; Fgf‐21, fibroblast growth factor 21; BDNF, brain‐derived neurotrophic factor; SEM, standard error of the mean; mRNA, messenger RNA.
Index in PubMed under a CC BY license. PMID: 40235186
Specific Publications For Anti-Fibroblast growth factor 21 FGF21 Antibody (A00802)
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