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Product Info Summary
| SKU: | M00564-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Fibronectin FN1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00564-1
BM4460 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Fibronectin FN1 Rabbit Monoclonal Antibody catalog # M00564-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibronectin FN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00564-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EID-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Fibronectin
Reactive Species
M00564-1 is reactive to FN1 in Human
Observed Molecular Weight
263 kDa
Calculated molecular weight
272.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00564-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
IHC: human liver cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Fibronectin expression in HepG2 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human liver cancer, using Fibronectin Antibody.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Effects of DBT on the expression of α-SMA, FN1, p-SMAD3 and TGF-β1 in rat lung tissue. α-SMA protein expression after (A) 28 and (B) 42 days of DBT administration. FN1 protein expression after (C) 28 and (D) 42 days of DBT administration; n = 4. (E) TGF-β1 and (F) p-SMAD3, n = 5. TGF-β1 protein expression after (G) 28 and (H) 42 days of DBT administration; n = 4 (G, H) , * P < 0.05 or ** P < 0.01; N.S. , not statistically significant, compared with the silica group.
Index in PubMed under a CC BY license. PMID: 40206091
Specific Publications For Anti-Fibronectin FN1 Rabbit Monoclonal Antibody (M00564-1)
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Customer Q&As
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16 Customer Q&As for Anti-Fibronectin FN1 Rabbit Monoclonal Antibody
Question
Would M00564-1 anti-Fibronectin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
As indicated on the product datasheet, M00564-1 anti-Fibronectin Rabbit Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-16
Question
Our lab were happy with the WB result of your anti-Fibronectin Rabbit Monoclonal antibody. However we have been able to see positive staining in retinal pigment epithelium extracellular space using this antibody. Is that expected? Could you tell me where is FN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
According to literature, retinal pigment epithelium does express FN1. Generally FN1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have FN1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cervix, Colon endothelium, Endometrial tumor, and Uterine endothelium, Pubmed ID: 17974005
Cartilage, Pubmed ID: 12127832
Cervix carcinoma, Pubmed ID: 17081983
Fibroblast, Pubmed ID: 2992939
Liver, Pubmed ID: 18318008, 19159218, 24275569
Mammary cancer, Pubmed ID: 3770189
Mammary carcinoma, Pubmed ID: 6304699
Peripheral blood T-cell, and Umbilical vein endothelial cell, Pubmed ID: 8406884, 3024962
Plasma, Pubmed ID: 2012601, 6630202, 14760718, 16335952
Retinal pigment epithelium, Pubmed ID: 16106752
Urine, Pubmed ID: 17614963
Boster Scientific Support
Answered: 2020-03-10
Question
I would like using your anti-Fibronectin Rabbit Monoclonal antibody for cell-substrate junction assembly studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
We appreciate your inquiry. This M00564-1 anti-Fibronectin Rabbit Monoclonal antibody is tested on hepg2 cell lysate. It is guaranteed to work for IHC, ICC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-26
Question
I see that the anti-Fibronectin Rabbit Monoclonal antibody M00564-1 works with ICC, what is the protocol used to produce the result images on the product page?
P. Dhar
Verified customer
Asked: 2019-11-19
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-19
Question
We have been able to see staining in human retinal pigment epithelium. Do you have any suggestions? Is anti-Fibronectin Rabbit Monoclonal antibody supposed to stain retinal pigment epithelium positively?
R. Williams
Verified customer
Asked: 2019-10-28
Answer
According to literature retinal pigment epithelium does express FN1. According to Uniprot.org, FN1 is expressed in tendon, retinal pigment epithelium, aortic endothelium, amygdala, cervix, colon endothelium, endometrial tumor
rc uterine endothelium, mammary cancer, fibroblast, plasma, peripheral blood t-cell umbilical vein endothelial cell, urine, cartilage, mammary carcinoma, cervix carcinoma, liver, among other tissues. Regarding which tissues have FN1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cervix, Colon endothelium, Endometrial tumor, and Uterine endothelium, Pubmed ID: 17974005
Cartilage, Pubmed ID: 12127832
Cervix carcinoma, Pubmed ID: 17081983
Fibroblast, Pubmed ID: 2992939
Liver, Pubmed ID: 18318008, 19159218, 24275569
Mammary cancer, Pubmed ID: 3770189
Mammary carcinoma, Pubmed ID: 6304699
Peripheral blood T-cell, and Umbilical vein endothelial cell, Pubmed ID: 8406884, 3024962
Plasma, Pubmed ID: 2012601, 6630202, 14760718, 16335952
Retinal pigment epithelium, Pubmed ID: 16106752
Urine, Pubmed ID: 17614963
Boster Scientific Support
Answered: 2019-10-28
Question
We ordered your anti-Fibronectin Rabbit Monoclonal antibody for ICC on aortic endothelium a few months ago. I am using human, and We intend to use the antibody for IHC next. I am looking for examining aortic endothelium as well as plasma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
I have checked the website and datasheets of our anti-Fibronectin Rabbit Monoclonal antibody and I see that M00564-1 has been validated on human in both ICC and IHC. Thus M00564-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-11
Question
I have attached the WB image, lot number and protocol we used for cervix using anti-Fibronectin Rabbit Monoclonal antibody M00564-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-08
Question
We are currently using anti-Fibronectin Rabbit Monoclonal antibody M00564-1 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The anti-Fibronectin Rabbit Monoclonal antibody (M00564-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix using anti-Fibronectin Rabbit Monoclonal antibody M00564-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-16
Question
I have a question about product M00564-1, anti-Fibronectin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Li
Verified customer
Asked: 2018-08-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00564-1 anti-Fibronectin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-07
Question
Is a blocking peptide available for product anti-Fibronectin Rabbit Monoclonal antibody (M00564-1)?
C. Moore
Verified customer
Asked: 2018-02-15
Answer
We do provide the blocking peptide for product anti-Fibronectin Rabbit Monoclonal antibody (M00564-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-02-15
Question
Do you have a BSA free version of anti-Fibronectin Rabbit Monoclonal antibody M00564-1 available?
L. Mitchell
Verified customer
Asked: 2016-12-26
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Fibronectin Rabbit Monoclonal antibody M00564-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-12-26
Question
I am interested in to test anti-Fibronectin Rabbit Monoclonal antibody M00564-1 on human cervix for research purposes, then I may be interested in using anti-Fibronectin Rabbit Monoclonal antibody M00564-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Collins
Verified customer
Asked: 2016-02-16
Answer
The products we sell, including anti-Fibronectin Rabbit Monoclonal antibody M00564-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-02-16
Question
Would anti-Fibronectin Rabbit Monoclonal antibody M00564-1 work for ICC with cervix?
G. Collins
Verified customer
Asked: 2015-01-08
Answer
According to the expression profile of cervix, FN1 is highly expressed in cervix. So, it is likely that anti-Fibronectin Rabbit Monoclonal antibody M00564-1 will work for ICC with cervix.
Boster Scientific Support
Answered: 2015-01-08
Question
Is this M00564-1 anti-Fibronectin Rabbit Monoclonal antibody reactive to the isotypes of FN1?
N. Carter
Verified customer
Asked: 2014-11-07
Answer
The immunogen of M00564-1 anti-Fibronectin Rabbit Monoclonal antibody is A synthesized peptide derived from human Fibronectin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-11-07
Question
I was wanting to use your anti-Fibronectin Rabbit Monoclonal antibody for ICC for human cervix on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix identification?
E. Evans
Verified customer
Asked: 2013-07-16
Answer
It shows on the product datasheet, M00564-1 anti-Fibronectin Rabbit Monoclonal antibody has been validated for IHC, ICC on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-16
