Fibronectin Antibodies

Fibronectin (FN1)

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.

Involved in osteoblast compaction throughout the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Gene Information

Human
Gene Name:	FN1
Uniprot:	P02751
Entrez:	2335

Family

Belongs to:
No superfamily

Alternative Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

Molecular Weight

Mass (kDA):
272.32 kDA

Genomic Context

Human
Location:	2q35
Sequence:	2; NC_000002.12 (215360440..215436167, complement)

Tissue Specificity

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.

Subcellular Localizations

Secreted, extracellular space, extracellular matrix.

Diseases

• Malignant Neoplasms
• Neoplasms
• Tissue Adhesions
• Carcinoma
• Cell Invasion
• Neoplasm Metastasis
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Neoplasm Invasiveness
• Fibrosis
• Inflammation
• Malignant Squamous Cell Neoplasm
• Thyroid Neoplasm

• Malignant Paraganglionic Neoplasm
• Malignant Neoplasm Of Lung
• Lung Neoplasms
• Cell Transformation, Neoplastic
• Tumor Angiogenesis
• Adenocarcinoma
• Malignant Neoplasm Of Ovary

Interacting Proteins

• COL1A1
• COL5A1
• CTGF
• DCN
• FBN1

• FBN2
• FYN
• LOX
• LPA
• SCGB1A1

• TGM2
• THBS1
• TNFAIP6
• UBQLN1
• VHL

Pathways

• Cell Adhesion
• Pathogenesis
• Cell Migration
• Cell Proliferation
• Cell Cycle
• Angiogenesis
• Aging
• Cell Motility
• Regeneration
• Cell Differentiation
• Cell-matrix Adhesion
• Immune Response
• Osteoblast Differentiation

• Embryo Development
• Epithelial To Mesenchymal Transition
• Brain Development
• Inflammatory Response
• Wound Healing
• Senescence
• Secretion

PTMs

• Phosphorylation
• Glycosylation

• Sulphation
• Cleavage

• Methylation

Research Areas

• Cancer
• Cellular Markers
• Mesenchymal Stem Cell Markers
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/FN1

Boster Bio Anti-Fibronectin FN1 Monoclonal Antibody

In this article, we'll look at the Boster Bio Anti-Fibronectin 1 FN1 Monoclonal Antibody and its uses. We'll also go over the Boster Bio ELISA Kits, and their validation and use. Continue reading to find out more about the numerous advantages this antibody offers. Also, we'll cover some of the other options that are available. In the end you'll use it in the most diverse settings.

Boster Bio Anti-Fibronectin 1 Monoclonal Antibody

The anti-Fibronectin protein is expressed in the extracellular matrix and on the cell surface, and Boster Bio Anti-Fibronectin Fn1 Monoclonal Immunoglobulin G (IgG) conjugated to Mouse has been extensively tested in Western Blot, ICC, and immunohistochemistry. It is also an excellent choice for studies on chronic inflammation, such as arthritis and osteoarthritis.

The antibody was tested in various cell lines including human fibroblasts, osteoblasts, human cells, and human fibroblasts. Western blots as well as RNA sequencing were done as previously described. RT-PCR as well as the enzyme-linked immunosorbent test (ELISA) were also used to determine if the antibody was effective under the conditions of the study.

Boster Bio ELISA Kits

Boster Bio creates ELISA Kits to detect biomarkers within immunology, cancer, neurodevelopment neurosciences, inflammation, and biomarkers in neurosciences, developmental biology cancer, and other fields. Boster Bio offers more than 12,000 antibodies, all of which have been tested against more than 250 different tissues. They are able to be used in a variety of applications and have sensitive levels as low as picograms. Tebu-bio distributes their immunological components.

The Human FN1 Kits for ELISA contain a standard containing 200 to 250 kDa of FN1 protein. Human FN1 is encoded in the chromosome 8 region and is associated with the process of cellular transformation, tumorigenesis, and other functions. FN1 is involved with the development of tumors, cellular transformation, and rearrangement of chromosome 8, in certain types of cancer. The human FN1 standard is a purified peptide derived from the plasma of rat.

Boster's Picokine Human Precoated ELISA Kit has a high sensitivity and significantly shorter run time. There are 96 wells included in the kit, as well as removable strips. This ELISA kit contains Recombinant Human FN1 and has no detectable cross-reactivity with other proteins. It contains recombinant Human FN1 with immunogen for a highly specific test.

Boster Bio FN1 ELISA kits can be used for the quantitative analysis of protein levels in different biological samples. They are suitable for the detection of FN1 in samples that are undiluted, such as serum and plasma. The kits are available in multiple formats, ranging from a basic 96-well plate to high-end, highly sensitive ELISA kits. Boster Bio is the best place to find an excellent FN1 Kit.

Validation

A recent study evaluated the FN1 marker as an epitope to fibronectin the vivo. It was shown that the lack of this marker did not impact the therapeutic effect of EVs of hepatocytes. To assess the deposition of collagen in liver tissues, Sirius red staining is employed. After six weeks of treatment with oil or CCl4 mice, they were then given EVDFN1 1 i.p. The last two weeks, mice were treated with CCl4 or oil for 6 weeks. After that, they received EVDFN1 1 i.p. The expression of fibrosis-related genes and cell cycle genes in hepatic cells was determined using qRT-PCR.

It is a vital component of Hepatocyte EVs and is responsible for EV binding to cells that are targeted by RGD (integrin)-dependent mechanisms. Additionally, it facilitates an endocytic system that can maintain a low pH, which prevents macropinocytosis. In addition, FN1 was found to be ineffective when it comes to binding EVs with caveolin and heparin-like molecules, which play essential roles in EV uptake.

FN1 was also detected in hepatocyte conditioned media. Prior to this, it was believed that FN1 was an EV-associated protein. ELISA was used to investigate FN1 immunoreactivity. Furthermore, FN1 immunoreactivity was found to be in line with flotillin-1 following high-speed centrifugation. The results also showed that ultracentriguation caused an almost complete loss of FN1 from supernatants, but not FN1 in pellets.

ELISA (enzyme-linked immunosorbent assay) was used to determine FN1 expression in culture supernatants and large vesicle cells. LifeSpan BioSciences manufactures ELISA kits that measure FN1 activity in cell culture. A number of mass spectrometry-based sequencing analyses have revealed the structure of FN1 and discovered that it is primarily associated with a peripheral membrane. Proteinase K digestion is required to fully digest FN1-expressing cells.

Applications

The FN1 marker is part of the plasma that contains an insoluble component known as fibronectin. It is a part of the ECM and is involved in ECM composition in a variety of tissue-specific ways. It is produced by the fibroblasts. It is a heterodimeric proteins with variable quantities of EDA and EDB domains. Its complex structure allows it to perform many biological functions and can dynamically adapt to changes in its environment.

FN1 is expressed in various tissues including stromal cells and tumors. Cancer cells are exposed to an extracellular matrix (ECM) when they invade the new environment. The cancer cells receive signals that determine their fate in this environment. For instance, FN1 is crucial for maintaining a balance between proliferation and apoptosis. If cancer cells aren't capable of communicating with the new ECM and stay in a state of dormancy. The new ECM allows cancer cells to communicate with it in order to regulate cell proliferation and die. This allows them to shrink their size. In some studies, the FN1 marker was discovered to be the primary driver for tumor progression.

The FN1 gene may also be connected with the development of fibrils without the involvement of integrins. This process is irreversible and can differentiate between fibrillar and liquid cancer cells that express FN1. Although the precise mechanism through that FN1 interacts with integrins isn't well understood, this process has been utilized as a way to distinguish between the two. These studies have led to a deeper understanding of integrin and FN1 interaction in cancer and have contributed to improving our understanding of the functions of integrins.

The FN1 protein is a part in extracellular matrix, plays an important role in renal fibrosis. The protein may be an important factor in leukocyte migration and can also stimulate T cell accumulation. Furthermore, in a new study, FN1 expression was associated with a decrease in GFR in patients suffering from DN. The FN1 marker was found to be in correlation with the levels of C3, which is a component of the complement system.