Anti-AREB6/ZEB1 Antibody Picoband™

ZEB1 antibody

Boster Bio Anti-AREB6/ZEB1 Antibody Picoband™ catalog # A00548-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: A00548-2
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-AREB6/ZEB1 Antibody Picoband™

View all ZEB1 Antibodies

SKU/Catalog Number

A00548-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AREB6/ZEB1 Antibody Picoband™ catalog # A00548-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AREB6/ZEB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00548-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ZEB1, which shares 94.9% and 100% amino acid (aa) sequence identity with rat ZEB1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00548-2 is reactive to ZEB1 in Human

Applications

A00548-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

200 kDa

Calculated molecular weight

124.074kDa

Background of ZEB1

ZEB1 (Zinc Finger E Box-Binding Homeobox 1), also called TCF8, NIL2A or DELTA-EF1, is a protein that in humans is encoded by the ZEB1 gene. Fluorescence in situ hybridization localized the ZEB1 gene to chromosome 10p11.2. Krafchak et al. (2005) demonstrated a complex (core plus secondary) binding site for TCF8 in the promoter of the COL4A3 gene, mutant in Alport syndrome and which encodes collagen type IV alpha-3. They detected expression of TCF8 in cornea. Nishimura et al. (2006) found that delta-Ef1 was upregulated during differentiation in a mouse smooth muscle cell (SMC) line.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ZEB1 (Source: Uniprot.org, NCBI)

Gene Name

ZEB1

Full Name

Zinc finger E-box-binding homeobox 1

Weight

124.074kDa

Superfamily

delta-EF1/ZFH-1 C2H2-type zinc-finger family

Alternative Names

AREB6; AREB6MGC133261; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; Negative regulator of IL2; NIL-2-A zinc finger protein; NIL2A; NIL-2-A; posterior polymorphous corneal dystrophy 3; PPCD3; TCF8; TCF-8; TCF8BZP; transcription factor 8 (represses interleukin 2 expression); Transcription factor 8; ZEB; ZEB1; ZFHEP; ZFHX1A; zinc finger E-box binding homeobox 1; zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein ZEB1 AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A zinc finger E-box binding homeobox 1 zinc finger E-box-binding homeobox 1|delta-crystallin enhancer binding factor 1|negative regulator of IL2|posterior polymorphous corneal dystrophy 3|transcription factor 8 (represses interleukin 2 expression)|zinc finger homeodomain enhancer-binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ZEB1, check out the ZEB1 Infographic

ZEB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ZEB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00548-2 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members

MiR-181a contributes gefitinib resistance in non-small cell lung cancer cells by targeting GAS7

Wang Q,Shang J,Zhang Y,Zhou Y,Tang L.MiR-451a restrains the growth and metastatic phenotypes of papillary thyroid carcinoma cells via inhibiting ZEB1.Biomed Pharmacother.2020 Jul;127:109901.doi:10.1016/j. biopha.2020.109901.Epub 2020 Apr 23.PMID:32335297.
Species: Human,Mouse
A00548-2 usage in article: APP:IF, SAMPLE:SW579 CELL AND B-CPAP CELL, DILUTION:1:500

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5 Customer Q&As for Anti-AREB6/ZEB1 Antibody Picoband™

Question

I am looking for using your anti-AREB6/ZEB1 antibody for positive regulation of neuron differentiation studies. Has this antibody been tested with western blotting on human hek293 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-12-26

Answer

We appreciate your inquiry. This A00548-2 anti-AREB6/ZEB1 antibody is validated on human hek293 whole cell lysates. It is guaranteed to work for Flow Cytometry, IF, IHC-P, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-12-26

Question

We have been able to see staining in human myometrium. What should we do? Is anti-AREB6/ZEB1 antibody supposed to stain myometrium positively?

Verified Customer

Verified customer

Asked: 2017-09-21

Answer

From what I have seen in literature myometrium does express ZEB1. From what I have seen in Uniprot.org, ZEB1 is expressed in myometrium, brain thalamus, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have ZEB1 expression, here are a few articles citing expression in various tissues:
Brain, and Thalamus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2017-09-21

Question

We ordered your anti-AREB6/ZEB1 antibody for IF on brain thalamus in the past. I am using human, and I plan to use the antibody for Flow Cytometry next. I would like examining brain thalamus as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2017-07-24

Answer

I looked at the website and datasheets of our anti-AREB6/ZEB1 antibody and it appears that A00548-2 has been tested on human in both IF and Flow Cytometry. Thus A00548-2 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-07-24

Question

We are currently using anti-AREB6/ZEB1 antibody A00548-2 for human tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-09

Answer

The anti-AREB6/ZEB1 antibody (A00548-2) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-09

Question

My team were satisfied with the WB result of your anti-AREB6/ZEB1 antibody. However we have been able to see positive staining in brain thalamus nucleus using this antibody. Is that expected? Could you tell me where is ZEB1 supposed to be expressed?

R. Li

Verified customer

Asked: 2015-10-19

Answer

From literature, brain thalamus does express ZEB1. Generally ZEB1 expresses in nucleus. Regarding which tissues have ZEB1 expression, here are a few articles citing expression in various tissues:
Brain, and Thalamus, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2015-10-19

Order DetailsPrice
A00548-2

100μg

$370
A00548-2-10ug

10μg sample (liquid)

$99
A00548-2-Biotin

100 μg Biotin conjugated

$570
A00548-2-Cy3

100 μg Cy3 conjugated

$570
A00548-2-Dylight488

100 μg Dylight488 conjugated

$570
A00548-2-Dylight550

100 μg Dylight550 conjugated

$570
A00548-2-Dylight594

100 μg Dylight594 conjugated

$570
A00548-2-FITC

100 μg FITC conjugated

$570
A00548-2-HRP

100 μg HRP conjugated

$570
A00548-2-APC

100 μg APC conjugated

$670
A00548-2-PE

100 μg PE conjugated

$670
A00548-2-carrier-free

Carrier Free

$370

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