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- Table of Contents
ZEB1 antibody (A00548-2) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Flow Cytometry analysis of K562 cells using anti-ZEB1 antibody (A00548-2).
Overlay histogram showing K562 cells stained with A00548-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB1 Antibody (A00548-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing K562 cells stained with A00548-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB1 Antibody (A00548-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-AREB6/ZEB1 Antibody Picoband®
ZEB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ZEB1 antibody was positively detected by WB in:
human 293T whole cell, human PC-3 whole cell, human HeLa whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ZEB1 antibody was positively detected by IHC in:
human glioma tissue, human melanoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ZEB1 antibody was positively detected by ICC/IF in:
U20S cell
ZEB1 antibody was positively detected by FC in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-AREB6/ZEB1 Antibody Picoband®
View all ZEB1 Antibodies
View all ZEB1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ZEB1, which shares 94.9% and 100% amino acid (aa) sequence identity with rat ZEB1.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
200 kDa
Calculated Molecular Weight
124.1 kDa
Background of ZEB1
ZEB1 (Zinc Finger E Box-Binding Homeobox 1), also called TCF8, NIL2A or DELTA-EF1, is a protein that in humans is encoded by the ZEB1 gene. Fluorescence in situ hybridization localized the ZEB1 gene to chromosome 10p11.2. Krafchak et al. (2005) demonstrated a complex (core plus secondary) binding site for TCF8 in the promoter of the COL4A3 gene, mutant in Alport syndrome and which encodes collagen type IV alpha-3. They detected expression of TCF8 in cornea. Nishimura et al. (2006) found that delta-Ef1 was upregulated during differentiation in a mouse smooth muscle cell (SMC) line.
Cite This Product
Anti-AREB6/ZEB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00548-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Mouse
95%
High Homology
Rat
95%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ZEB1 antibody
Immunohistochemistry (IHC) protocol for ZEB1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ZEB1 antibody
Flow Cytometry (FC) protocol for ZEB1 antibody
Publications
Application:
Species:
Enhanced ITGA3 expression induced by helicobacter pylori infection facilitates gastric cancer progression via NF-κB and Smad4
Published: 2026-06-04
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 4.70
Species: Human
PMID: 42240927
STAT5A modulates gastric cancer progression via upregulation of CD44
Published: 2023-05-15
Journal: GENOMICS
Application: WB
Impact Factor: 4.40
Species: Human
PMID: 37196931
MALAT-1 regulates the AML progression by promoting the m6A modification of ZEB1
Published: 2023-02-22
Journal: ACTA BIOCHIMICA POLONICA
Application: WB
Impact Factor: 1.70
Species: Human
PMID: 36812496
Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells
Published: 2022-12-10
Journal: TOXICOLOGY
Application: WB
Impact Factor: 4.57
Species: Human
PMID: 36513242
MiR-451a restrains the growth and metastatic phenotypes of papillary thyroid carcinoma cells via inhibiting ZEB1
Published: 2020-04-23
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: IF
Impact Factor: 4.55
Species: Human
PMID: 32335297
All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members
Published: 2020-01-10
Journal: Genes & Diseases
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 34179313
Identification of FOXM1 as a specific marker for triple‑negative breast cancer
Published: 2018-10-19
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: IF
Impact Factor: 3.33
Species: Human
PMID: 30365046
MiR-181a contributes gefitinib resistance in non-small cell lung cancer cells by targeting GAS7
Published: 2017-12-18
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.47
Species: Human
PMID: 29269300
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Product Promise
We promise Anti-AREB6/ZEB1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00548-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026