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- Table of Contents
EPCAM antibody (PB10059) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of CACO-2 cells using anti-EPCAM antibody (PB10059).
Overlay histogram showing CACO-2 cells stained with PB10059 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EPCAM Antibody (PB10059, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing CACO-2 cells stained with PB10059 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EPCAM Antibody (PB10059, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-EpCAM Antibody Picoband®
EPCAM antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
EPCAM antibody was positively detected by WB in:
human CACO-2 whole cell, human MCF-7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
EPCAM antibody was positively detected by IHC in:
human colon cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
EPCAM antibody was positively detected by IF in:
human rectal cancer tissue
EPCAM antibody was positively detected by FC in:
CACO-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Immunofluorescence
5μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106cells
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-EpCAM Antibody Picoband®
View all EPCAM Antibodies
View all EPCAM Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human EPCAM, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by sixteen amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35-40 kDa
Calculated Molecular Weight
34.9 kDa
Background of EPCAM
Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Cite This Product
Anti-EpCAM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10059)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
81%
Moderate Homology
Rabbit
77%
Moderate Homology
Cow
72%
Moderate Homology
Sheep
72%
Moderate Homology
Mouse
65%
Low homology
Dog
65%
Low homology
Rat
63%
Low homology
Zebrafish
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for EPCAM antibody
Immunohistochemistry (IHC) protocol for EPCAM antibody
Immunofluorescence (IF) protocol for EPCAM antibody
Flow Cytometry (FC) protocol for EPCAM antibody
Publications
Application:
Species:
Epithelial cell adhesion molecule promotes breast cancer resistance protein-mediated multidrug resistance in breast cancer by inducing partial epithelial–mesenchymal transition
Published: 2021-03-24
Journal: CELL BIOLOGY INTERNATIONAL
Application: IF
Impact Factor: 3.61
Species: Human
PMID: 33760350
Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage
Published: 2017-11-28
Journal: Stem Cell Research & Therapy
Application: IF
Impact Factor: 4.21
Species: Human
PMID: 29179771
Deformability and size-based cancer cell separation using an integrated microfluidic device
Published: 2015-08-25
Journal: ANALYST
Application: ICC
Impact Factor: 4.11
Species: Human
PMID: 26366443
Review
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1 Review For Anti-EpCAM Antibody Picoband®
Immunoflorescence Review for Anti-EpCAM Antibody
| SKU | PB10059 |
|---|---|
| Application | Immunofluorescence (IF) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human rectal |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC
kit from Boster Bio, (SA1022 |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
A. Dayal
Verified customer
Submitted 2019-05-25
Product Promise
We promise Anti-EpCAM Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PB10059
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026