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Product Info Summary
| SKU: | M00276 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD326 Purified EPCAM Monoclonal Antibody
SKU/Catalog Number
M00276
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD326 Purified EPCAM Monoclonal Antibody (Catalog# M00276). Tested in Flow Cytometry, IHC-P, IP, WB, IHC-F, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD326 Purified EPCAM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00276)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
VU-1D9
Isotype
Mouse IgG1
Immunogen
Small cell lung carcinoma cell line H69. The mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.
Reactive Species
M00276 is reactive to EPCAM in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
34.9 kDa
Background of EPCAM
CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00276 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin.
Immunocytochemistry: 4 μg/ml.
Western blotting: 1-4 μg/ml, non-reducing conditions.
Flow cytometry: 1-3 μg/ml.
Immunoprecipitation: 1-2 μg / 100-500 μg of protein in 1 ml lysate.
Validation Images & Assay Conditions
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Separation of MCF-7 cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) of cell lines stained using anti-human CD326 (VU-1D9) purified antibody (concentration in sample 6 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of MCF-7 cells stained using anti-human CD326 (VU-1D9) purified antibody (concentration in sample 6 µg/ml) GAM APC.
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Immunohistochemistry staining of human prostate (paraffin sections) using anti-CD326 (VU-1D9). Commercially tested by LifeSpan BioSciences.
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Western blotting analysis of human CD326 using mouse monoclonal antibody VU-1D9 on lysates of MCF-7 cells and HEK293T cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD326 was detected around 40 kDa.
Specific Publications For Anti-Hu CD326 Purified EPCAM Monoclonal Antibody (M00276)
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