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FN1 antibody (M00564-3) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 43 Publications

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m00564 3 fibronectin primary antibodies ihc testing 2

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IHC analysis of Fibronectin using anti-Fibronectin antibody (M00564-3).
Fibronectin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Fibronectin Antibody (M00564-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-Fibronectin Rabbit Monoclonal Antibody

FN1 antibody

43 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

FN1 antibody was positively detected by WB in:

human U-87MG whole cell, human HepG2 whole cell, rat liver tissue, mouse liver tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

FN1 antibody was positively detected by IHC in:

human colorectal adenocarcinoma tissue, human liver cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Fibronectin Rabbit Monoclonal Antibody
View all FN1 Antibodies

Form

Liquid

Uniprot

P02751

Gene ID

2335

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

26F78

Isotype

IgG

Immunogen

A synthesized peptide derived from human Fibronectin

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

272 kDa

Calculated Molecular Weight

272.3 kDa

Cite This Product

Anti-Fibronectin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00564-3)

Protocols

Western Blot protocol for FN1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for FN1 antibody

Review Protocol

Publications

Development of a Shapley Additive Explanations (SHAP)-Interpretable Model for Cardiovascular Risk Stratification in Type 2 Diabetes Mellitus: Implications of MDA-Modified Protein Adducts and Autoantibodies

Published: 2026-08-13

Journal: ACS Omega

Application: IP

Impact Factor: 5.20

Species: Human

PMID: 42662675

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Free Secondary Antibody

Anti-Fibronectin Rabbit Monoclonal Antibody

FN1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Development of a Shapley Additive Explanations (SHAP)-Interpretable Model for Cardiovascular Risk Stratification in Type 2 Diabetes Mellitus: Implications of MDA-Modified Protein Adducts and Autoantibodies

Metabolic reprogramming by baicalein disrupts fibroblast–macrophage crosstalk in idiopathic pulmonary fibrosis

Glucosamine alleviates liver fibrosis through modulation of sinusoidal blood flow and hepatic immune responses

Establishment and optimization of a novel mouse model of hyperuricemic nephropathy

“Qi Nan” agarwood restores podocyte autophagy in diabetic kidney disease by targeting EGFR signaling pathway

Role and mechanism of Circ-PDE7B in the formation of keloid

Therapeutic mechanism of baicalein in peritoneal dialysis-associated peritoneal fibrosis based on network pharmacology and experimental validation

Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork

Effects of Ocular Hypertension on Cytoskeleton and Stiffness of Trabecular Meshwork Cells in Rats

Enzymatic self-assembly nanofibers anchoring mesenchymal stem cells induce cell spheroids and amplify paracrine function for myocardial infarction therapy

An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties

Effect of Acellular Amnion With Increased TGF-β and bFGF Levels on the Biological Behavior of Tenocytes

Injured tubular epithelial cells activate fibroblasts to promote kidney fibrosis through miR-150-containing exosomes

Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition in Lens Epithelial Cells through Multiple Pathways

Fructose-1,6-bisphosphate aldolase encoded by a core gene of Mycoplasma hyopneumoniae contributes to host cell adhesion

C4orf7 modulates osteogenesis and adipogenesis of human periodontal ligament cells

Characterization of pathogenic roles of two Erysipelothrix rhusiopathiae surface proteins

Quantitative Studies of Endothelial Cell Fibronectin and Filamentous Actin (F-Actin) Coalignment in Response to Shear Stress

TNF-α-induced NF-κB activation promotes myofibroblast differentiation of LR-MSCs and exacerbates bleomycin-induced pulmonary fibrosis

SnoN upregulation ameliorates renal fibrosis in diabetic nephropathy

Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathway

Decellularized ovine arteries as biomatrix scaffold support endothelial of mesenchymal stem cells

Blockade of Orai1 Store-Operated Calcium Entry Protects against Renal Fibrosis

Overexpression of SDF-1 activates the NF-κB pathway to induce epithelial to mesenchymal transition and cancer stem cell-like phenotypes of breast cancer cells

RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells

Effects of transforming growth factor-β2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells

Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathy

MAGEC2, an epithelial-mesenchymal transition inducer, is associated with breast cancer metastasis

Caveolin-1 Is Up-Regulated by GLI1 and Contributes to GLI1-Driven EMT in Hepatocellular Carcinoma

Extracellular Matrix of Mechanically Stretched Cardiac Fibroblasts Improves Viability and Metabolic Activity of Ventricular Cells

Probucol attenuates ethanol-induced liver fibrosis in rats by inhibiting oxidative stress, extracellular matrix protein accumulation and cytokine production

Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates

Epithelial–Mesenchymal Transition (EMT) Induced by TNF-α Requires AKT/GSK-3β-Mediated Stabilization of Snail in Colorectal Cancer

Nodal promotes aggressive phenotype via Snail-mediated epithelial–mesenchymal transition in murine melanoma

EGCG inhibits CTGF expression via blocking NF-κB activation in cardiac fibroblast

Quantitative determination of matrix Gla protein (MGP) and BMP-2 during the osteogenic differentiation of human periodontal ligament cells

Inhibition of Calcium–Calmodulin-Dependent Kinase II Suppresses Cardiac Fibroblast Proliferation and Extracellular Matrix Secretion

Microplasmin Degrades Fibronectin and Laminin at Vitreoretinal Interface and Outer Retina during Enzymatic Vitrectomy

Mechanical stretch promotes proliferation of rat bone marrow mesenchymal stem cells

Rosiglitazone Prevents Advanced Glycation End Products–Induced Renal Toxicity Likely through Suppression of Plasminogen Activator Inhibitor-1

A New ATP-Sensitive Potassium Channel Opener Protects the Kidney from Hypertensive Damage in Spontaneously Hypertensive Rats

Role of impaired peritubular capillary and hypoxia in progressive interstitial fibrosis after 56 subtotal nephrectomy of rats

Protective effects of blocking renin-angiotensin system on the progression of renal injury in glomerulosclerosis.

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