Anti-FMR1 Antibody

Product Name

Anti-FMR1 Antibody

View all FMR1 Antibodies

SKU/Catalog Number

A00192

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FMR1 Antibody (Catalog # A00192). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

FMR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-FMR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00192)

Host

Rabbit

Contents

FMR1 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FMR1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FMR1. The immunogen is located within the last 50 amino acids of FMR1.

Cross-reactivity

FMR1 antibody is human, mouse and rat reactive. Multiple isoforms of FMR1 are known to exist.

Reactive Species

A00192 is reactive to FMR1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

71.2 kDa

Background of FMR1

Fragile X syndrome is a frequent form of inherited mental retardation caused by functional loss of the fragile X mental retardation protein, FMR1, also known as FMRP (1). FMR1 binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm (2). A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome (1). Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1) (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00192 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FMR1 antibody can be used for detection of FMR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information FMR1

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis