Product Info Summary
SKU: | A00192-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FMRP FMR1 Antibody
SKU/Catalog Number
A00192-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-FMRP FMR1 Antibody catalog # A00192-1. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FMRP FMR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00192-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Flt-3 around the phosphorylation site of Y969.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00192-1 is reactive to FMR1 in Human, Mouse, Rat
Applications
A00192-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
71.174kDa
Background of FMRP
FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Sekine S et,al. (2008) J Immunol. ;180(12):8126-34
Pratz K et,al. (2008) Leuk Lymphoma. 2008 ;49(5):852-63.
Al Shaer L et,al. (2008) Br J Haematol. 141(4):483-93.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000-1:10,000
ICC: 1:100-1:500
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of FMR1 using Anti-FMRP FMR1 Antibody (A00192-1).
ICC staining FMRP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-FMRP FMR1 Antibody A00192-1
Western blot analysis of FMRP on different cells lysates using anti-FMRP antibody at 1/500 dilution. Positive control:Lane 1: Hela
Lane 2: K562
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For FMR1 (Source: Uniprot.org, NCBI)
Gene Name
FMR1
Full Name
Synaptic functional regulator FMR1
Weight
71.174kDa
Superfamily
FMR1 family
Alternative Names
FMRPFRAXAPOF; fragile X mental retardation 1 protein; fragile X mental retardation 1; MGC87458; POF1; premature ovarian failure 1; Protein FMR-1 FMR1 FMRP, FRAXA, POF, POF1 FMRP translational regulator 1 synaptic functional regulator FMR1|fragile X mental retardation 1|fragile X mental retardation protein 1|truncated FMRP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FMR1, check out the FMR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FMR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FMRP FMR1 Antibody (A00192-1)
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No publications found for A00192-1
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