Anti-FMRP/FMR1 Antibody Picoband®

FMRP antibody

Boster Bio Anti-FMRP/FMR1 Antibody Picoband® catalog # PB9703. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9703
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

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Product Name

Anti-FMRP/FMR1 Antibody Picoband®

View all FMRP Antibodies

SKU/Catalog Number

PB9703

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FMRP/FMR1 Antibody Picoband® catalog # PB9703. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FMRP/FMR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9703)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human FMRP, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9703 is reactive to FMR1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

71-73 kDa

Calculated molecular weight

71174 MW

Background of FMRP

FMR1  (fragile X mental retardation 1) is a human gene that codes for a protein called fragile X mentalretardation protein, or FMRP. This protein, most commonly found in the brain, is essential for normal cognitive development and female reproductive function. Mutations of this gene can lead to fragile X syndrome, mental retardation, premature ovarian failure, autism, Parkinson's disease, developmental delays and other cognitive deficits. The protein encoded by this gene binds RNA and is associated with polysomes. Additionally, the encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9703 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Positive Control

WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell, human K562 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
IHC: human seminoma testis tissue
ICC/IF: Hela cell

Validation Images & Assay Conditions

Gene/Protein Information For FMR1 (Source: Uniprot.org, NCBI)

Gene Name

FMR1

Full Name

Synaptic functional regulator FMR1

Weight

71174 MW

Superfamily

FMR1 family

Alternative Names

Fragile X mental retardation protein 1;FMRP;Protein FMR-1;FMR1; FMR1 FMRP, FRAXA, POF, POF1 FMRP translational regulator 1 synaptic functional regulator FMR1|fragile X mental retardation 1|fragile X mental retardation protein 1|truncated FMRP

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FMR1, check out the FMR1 Infographic

FMR1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FMR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-FMRP/FMR1 Antibody Picoband®

Question

We are currently using anti-FMRP/FMR1 antibody PB9703 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

A. Anderson

Verified customer

Asked: 2020-01-24

Answer

The anti-FMRP/FMR1 antibody (PB9703) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-24

Question

Would PB9703 anti-FMRP/FMR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

It shows on the product datasheet, PB9703 anti-FMRP/FMR1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-26

Question

I see that the anti-FMRP/FMR1 antibody PB9703 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-26

Question

My question regarding product PB9703, anti-FMRP/FMR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-01-01

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9703 anti-FMRP/FMR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-01

Question

I was wanting to use your anti-FMRP/FMR1 antibody for WB for human placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta identification?

L. Zhao

Verified customer

Asked: 2017-04-27

Answer

It shows on the product datasheet, PB9703 anti-FMRP/FMR1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-04-27

Order DetailsPrice
PB9703

100μg

$370
PB9703-10ug

10μg sample (liquid)

$99
PB9703-Biotin

100 μg Biotin conjugated

$570
PB9703-Cy3

100 μg Cy3 conjugated

$570
PB9703-Dylight488

100 μg Dylight488 conjugated

$570
PB9703-Dylight550

100 μg Dylight550 conjugated

$570
PB9703-Dylight594

100 μg Dylight594 conjugated

$570
PB9703-FITC

100 μg FITC conjugated

$570
PB9703-HRP

100 μg HRP conjugated

$570
PB9703-APC

100 μg APC conjugated

$670
PB9703-PE

100 μg PE conjugated

$670
PB9703-iFluor647

100 μg iFluor647 conjugated

$670
PB9703-carrier-free

Carrier Free

$370

More conjugates/formats

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PB9703
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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