Click image to see more details
Product Info Summary
| SKU: | A02590-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-AF4 AFF1 Antibody
SKU/Catalog Number
A02590-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AF4 AFF1 Antibody (Catalog# A02590-1). Tested in IHC, ICC, IF, WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AF4 AFF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02590-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human AF4. Immunogen sequence location: 1-50
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02590-1 is reactive to AFF1 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
131.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02590-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AF4 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-AF4 AFF1 Antibody (A02590-1)
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Customer Q&As
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7 Customer Q&As for Anti-AF4 AFF1 Antibody
Question
Would A02590-1 anti-AF4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
As indicated on the product datasheet, A02590-1 anti-AF4 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-AF4 antibody A02590-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-30
Question
We are currently using anti-AF4 antibody A02590-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The anti-AF4 antibody (A02590-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-26
Question
Will anti-AF4 antibody A02590-1 work for IHC with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
According to the expression profile of cervix carcinoma erythroleukemia, AFF1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-AF4 antibody A02590-1 will work for IHC with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-09-19
Question
Is a blocking peptide available for product anti-AF4 antibody (A02590-1)?
F. Collins
Verified customer
Asked: 2017-12-12
Answer
We do provide the blocking peptide for product anti-AF4 antibody (A02590-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-12-12
Question
I was wanting to use your anti-AF4 antibody for IHC for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?
R. Taylor
Verified customer
Asked: 2017-01-05
Answer
As indicated on the product datasheet, A02590-1 anti-AF4 antibody has been tested for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-05
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-AF4 antibody A02590-1. Please let me know if you require anything else.
G. Wu
Verified customer
Asked: 2015-11-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-11-24
