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Product Info Summary
| SKU: | A00962-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-FoxC1/2 Antibody
SKU/Catalog Number
A00962-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-FoxC1/2 Antibody catalog # A00962-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FoxC1/2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00962-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human FOXC1/2. AA range:151-200
Reactive Species
A00962-1 is reactive to FOXC1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
56.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00962-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4° overnight.3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of hela cells using FoxC1/2 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from RAW264.7 cells, using FOXC1/2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from Jurkat cells using FOXC1/2 antibody.
Specific Publications For Anti-FoxC1/2 Antibody (A00962-1)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-FoxC1/2 Antibody
Question
Is a blocking peptide available for product anti-FoxC1/2 antibody (A00962-1)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We do provide the blocking peptide for product anti-FoxC1/2 antibody (A00962-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-16
Question
Would anti-FoxC1/2 antibody A00962-1 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
According to the expression profile of leukemic t-cell, FOXC1 is highly expressed in leukemic t-cell. So, it is likely that anti-FoxC1/2 antibody A00962-1 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-08-22
Question
We want to test anti-FoxC1/2 antibody A00962-1 on rat leukemic t-cell for research purposes, then I may be interested in using anti-FoxC1/2 antibody A00962-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The products we sell, including anti-FoxC1/2 antibody A00962-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-31
Question
Is this A00962-1 anti-FoxC1/2 antibody reactive to the isotypes of FOXC1?
F. Bhatt
Verified customer
Asked: 2014-03-31
Answer
The immunogen of A00962-1 anti-FoxC1/2 antibody is Synthesized peptide derived from the Internal region of human FoxC1/2. at AA rangle: 120-200. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-03-31
