Anti-Forkhead box protein D3 FOXD3 Antibody

Boster Bio Anti-Forkhead box protein D3 FOXD3 Antibody catalog # A04495. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A04495
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Forkhead box protein D3 FOXD3 Antibody

See all FOXD3 products

SKU/Catalog Number

A04495

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Forkhead box protein D3 FOXD3 Antibody catalog # A04495. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Forkhead box protein D3 FOXD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04495)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from internal of human FOXD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04495 is reactive to FOXD3 in Human, Mouse

Applications

A04495 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:3000
IHC, 1:50-1:100

Validation Images & Assay Conditions

Gene/Protein Information For FOXD3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9UJU5

Gene ID

27022

Gene Name

FOXD3

Full Name

Forkhead box protein D3

Weight

47.63kDa

Alternative Names

Forkhead box protein D3 FOXD3 AIS1, Genesis, HFH2, VAMAS2 forkhead box D3 forkhead box protein D3|HNF3/FH transcription factor genesis

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FOXD3, check out the FOXD3 Infographic

FOXD3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FOXD3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A04495

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13 Customer Q&As for Anti-Forkhead box protein D3 FOXD3 Antibody

Question

I see that the anti-FOXD3 antibody A04495 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-20

Question

We need to test anti-FOXD3 antibody A04495 on human tibial nerve for research purposes, then I may be interested in using anti-FOXD3 antibody A04495 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

The products we sell, including anti-FOXD3 antibody A04495, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-20

Question

We are currently using anti-FOXD3 antibody A04495 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

The anti-FOXD3 antibody (A04495) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-11

Question

Is a blocking peptide available for product anti-FOXD3 antibody (A04495)?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

We do provide the blocking peptide for product anti-FOXD3 antibody (A04495). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-05

Question

Can you help my question with product A04495, anti-FOXD3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04495 anti-FOXD3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-29

Question

Would anti-FOXD3 antibody A04495 work on canine WB with chronic myeloid leukemia cell?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

Our lab technicians have not validated anti-FOXD3 antibody A04495 on canine. You can run a BLAST between canine and the immunogen sequence of anti-FOXD3 antibody A04495 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine chronic myeloid leukemia cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-15

Question

Will anti-FOXD3 antibody A04495 work for IHC with tibial nerve?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

According to the expression profile of tibial nerve, FOXD3 is highly expressed in tibial nerve. So, it is likely that anti-FOXD3 antibody A04495 will work for IHC with tibial nerve.

Boster Scientific Support

Answered: 2019-08-09

Question

See below the WB image, lot number and protocol we used for tibial nerve using anti-FOXD3 antibody A04495. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-18

Question

Do you have a BSA free version of anti-FOXD3 antibody A04495 available?

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FOXD3 antibody A04495 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-02

Question

I was wanting to use your anti-FOXD3 antibody for IHC for human tibial nerve on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human tibial nerve identification?

Verified Customer

Verified customer

Asked: 2019-02-28

Answer

It shows on the product datasheet, A04495 anti-FOXD3 antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human tibial nerve in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-28

Question

Is this A04495 anti-FOXD3 antibody reactive to the isotypes of FOXD3?

Verified Customer

Verified customer

Asked: 2018-09-24

Answer

The immunogen of A04495 anti-FOXD3 antibody is Synthesized peptide derived from internal of human FOXD3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-24

Question

Will A04495 anti-FOXD3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-05-25

Answer

It shows on the product datasheet, A04495 anti-FOXD3 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-05-25

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibial nerve using anti-FOXD3 antibody A04495. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-15

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A04495
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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