Anti-FoxP3 Purified Monoclonal Antibody

Product Name

Anti-FoxP3 Purified Monoclonal Antibody

View all FOXP3 Antibodies

SKU/Catalog Number

M00011-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FoxP3 Purified Monoclonal Antibody (Catalog# M00011-1). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-FoxP3 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00011-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

3G3

Isotype

Mouse IgG1 kappa

Immunogen

Full-length His-tagged recombinant murine FoxP3. The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells.

Reactive Species

M00011-1 is reactive to FOXP3 in Human, Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

47.2 kDa

Background of FOXP3

FoxP3 (forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00011-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 2 μg/ml.
Flow cytometry: 1-4 µg/ml. Intracellular staining.

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information FOXP3

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