This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Il17a antibody (A00421-2) performs well in IHC, WB, ELISA (Cap) and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 16 Publications
Filter:
IHC analysis of IL17 using anti-IL17 antibody (A00421-2).
IL17 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL17 Antibody (A00421-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IL17 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL17 Antibody (A00421-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Bundle Save
Anti-IL17/Il17a Antibody Picoband®
Il17a antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB, ELISA (Cap) |
Tested Applications
Il17a antibody was positively detected by WB in:
human Jurkat cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Il17a antibody was positively detected by IHC in:
mouse spleen tissue, rat spleen tissue, rat pancreas tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
ELISA (Cap)
1-5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Mouse
Tested
Recommend
Rat
Tested
Recommend
Product Information
Product Name
Anti-IL17/Il17a Antibody Picoband®
View all IL17A Antibodies
View all IL17A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL17 recombinant protein (Position: A26-A158).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa
Calculated Molecular Weight
17.5 kDa
Background of Il17a
Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Cite This Product
Anti-IL17/Il17a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00421-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
89%
Moderate Homology
Cow
68%
Low homology
Sheep
68%
Low homology
Rabbit
66%
Low homology
Monkey
62%
Low homology
Human
61%
Low homology
Dog
48%
Low homology
Zebrafish
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Il17a antibody
Immunohistochemistry (IHC) protocol for Il17a antibody
Publications
Application:
Species:
Gut microbiota-derived indoleacrylic acid ameliorates rheumatoid arthritis by preventing necroptosis of fibroblast-like synoviocytes and regulating macrophage polarization
Published: 2026-08-17
Journal: npj Biofilms and Microbiomes
Application: IHC,IF
Impact Factor: 11.40
Species: Mouse
Porphyromonas gingivalis induces intestinal inflammation through gingipain-dependent gut microbiome dysbiosis
Published: 2026-04-02
Journal: Microbiome
Application: IF
Impact Factor: 12.70
Species: Mouse
PMID: 41923122
Probiotic and antibacterial properties of recombinant Lactococcus lactis expressing the fusion antimicrobial peptides BMAP18-BSN37 in mice and chickens
Published: 2026-01-23
Journal: POULTRY SCIENCE
Application: WB
Impact Factor: 4.20
Species: Mouse
PMID: 41616534
An intelligent bacterial capture nanodrug enhances immunotherapy for implant-associated infections by inducing cuproptosis-like death in bacteria
Published: 2025-12-30
Journal: JOURNAL OF CONTROLLED RELEASE
Application: IHC
Impact Factor: 11.50
Species: Rat
PMID: 41478375
Targeting Talin1 to reprogram dendritic cell activation landscapes: A mechanistically grounded cell therapy for multiple sclerosis model
Published: 2025-08-22
Journal: Neurotherapeutics
Application: IHC
Impact Factor: 6.90
Species: Mouse
PMID: 40846592
Quercetin alleviates ulcerative colitis through inhibiting CXCL8-CXCR1/2 axis: a network and transcriptome analysis
Published: 2024-12-09
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.40
Species: Mouse
PMID: 39717557
Mechanic study based on untargeted metabolomics of Pi-pa-run-fei-tang on pepper combined with ammonia induced chronic cough model mice
Published: 2024-02-15
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38364934
Shikonin promotes ferroptosis in HaCaT cells through Nrf2 and alleviates imiquimod-induced psoriasis in mice
Published: 2023-10-29
Journal: CHEMICO-BIOLOGICAL INTERACTIONS
Application: WB
Impact Factor: 5.10
Species: Mouse,Human
Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration
Published: 2023-09-22
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: IHC
Impact Factor: 5.40
Species: Mouse
PMID: 37772372
Pi-Pa-Run-Fei-Tang alleviates lung injury by modulating IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB signaling pathway and balancing Th17 and Treg in murine model of OVA-induced asthma
Published: 2023-05-31
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 37268260
IL-23/IL-17 immune axis mediates the imiquimod-induced psoriatic inflammation by activating ACT1/TRAF6/TAK1/NF-κB pathway in macrophages and keratinocytes
Published: 2023-04-26
Journal: KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
Impact Factor: 3.30
Species: Mouse
PMID: 37098777
A Synbiotic Ameliorates Con A-Induced Autoimmune Hepatitis in Mice through Modulation of Gut Microbiota and Immune Imbalance
Published: 2023-01-28
Journal: MOLECULAR NUTRITION & FOOD RESEARCH
Application: IHC
Impact Factor: 5.20
Species: Mouse
PMID: 36708241
IL-17–induced HIF1α drives resistance to anti–PD-L1 via fibroblast-mediated immune exclusion
Published: 2022-04-07
Journal: JOURNAL OF EXPERIMENTAL MEDICINE
Application: IHC,IF
Impact Factor: 17.58
Species: Mouse
PMID: 35389431
Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats
Published: 2017-11-13
Journal: Scientific Reports
Application: IHC
Impact Factor: 4.26
Species: Rat
PMID: 29133889
Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice
Published: 2015-05-03
Journal: Evidence-based Complementary and Alternative Medicine
Application: WB
Impact Factor: 1.88
Species: Mouse
PMID: 26064167
Th17 cells are associated with the Th1/Th2‑cell balance during Echinococcus multilocularis infection
Published: 2014-04-24
Journal: Molecular Medicine Reports
Application: IHC
Impact Factor: 1.48
Species: Mouse
PMID: 24789110
Review
Have you used Anti-IL17/Il17a Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-IL17/Il17a Antibody Picoband® performs excellently in relevant applications for IHC, WB, ELISA (Cap). If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00421-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026