Anti-IL17/Il17a Antibody Picoband™

IL-17/IL-17A antibody

Boster Bio Anti-IL17/Il17a Antibody Picoband™ catalog # A00421-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 3 publication(s).

Product Info Summary

SKU: A00421-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-IL17/Il17a Antibody Picoband™

View all IL-17/IL-17A Antibodies

SKU/Catalog Number

A00421-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL17/Il17a Antibody Picoband™ catalog # A00421-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL17/Il17a Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00421-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse IL17 recombinant protein (Position: A26-A158).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00421-2 is reactive to Il17a in Human, Mouse, Rat

Applications

A00421-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of IL-17/IL-17A

Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Il17a (Source: Uniprot.org, NCBI)

Uniprot ID

Q62386

Gene ID

16171

Gene Name

Il17a

Full Name

Interleukin-17A

Weight

17.49kDa

Superfamily

IL-17 family

Alternative Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A Il17a|Ctl, Ctla, Ctla-8, Ctla8, IL-, IL-17, IL-17A, Il, Il17|interleukin 17A|interleukin-17A|cytotoxic T-lymphocyte-associated antigen 8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Il17a, check out the Il17a Infographic

Il17a infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Il17a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

A00421-2 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wu Y, Li J, Yan B, Zhu Y, Liu X, Chen M, Li D, Lee CC, Yang X, Ma P. Sci Rep. 2017 Nov 13;7(1):15469. doi: 10.1038/s41598-017-15533-z. Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in femal...

Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...

Li Xn, Pan X, Qiu D. Int J Clin Exp Med. 2014 Dec 15;7(12):5324-9. Ecollection 2014. Imbalances Of Th17 And Treg Cells And Their Respective Cytokines In Copd Patients By Disease Stage.

Have you used Anti-IL17/Il17a Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-IL17/Il17a Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-IL17/Il17a Antibody Picoband™

Question

We have observed staining in human palpebral conjunctiva. Do you have any suggestions? Is anti-IL17/Il17a antibody supposed to stain palpebral conjunctiva positively?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

From what I have seen in literature palpebral conjunctiva does express IL17A. From what I have seen in Uniprot.org, IL17A is expressed in palpebral conjunctiva, t-cell, among other tissues. Regarding which tissues have IL17A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 7499828, 14574404

Boster Scientific Support

Answered: 2020-03-03

Question

Does A00421-2 anti-IL17/Il17a antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

As indicated on the product datasheet, A00421-2 anti-IL17/Il17a antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-02

Question

We are currently using anti-IL17/Il17a antibody A00421-2 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

The anti-IL17/Il17a antibody (A00421-2) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-27

Question

See attached the WB image, lot number and protocol we used for palpebral conjunctiva using anti-IL17/Il17a antibody A00421-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-12

Question

My question regarding product A00421-2, anti-IL17/Il17a antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00421-2 anti-IL17/Il17a antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-10

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for palpebral conjunctiva using anti-IL17/Il17a antibody A00421-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-03

Question

Is a blocking peptide available for product anti-IL17/Il17a antibody (A00421-2)?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

We do provide the blocking peptide for product anti-IL17/Il17a antibody (A00421-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-12

Question

Does anti-IL17/Il17a antibody A00421-2 work for ELISA with palpebral conjunctiva?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

According to the expression profile of palpebral conjunctiva, IL17A is highly expressed in palpebral conjunctiva. So, it is likely that anti-IL17/Il17a antibody A00421-2 will work for ELISA with palpebral conjunctiva.

Boster Scientific Support

Answered: 2019-07-24

Question

I am interested in using your anti-IL17/Il17a antibody for positive regulation of interleukin-6 secretion studies. Has this antibody been tested with western blotting on human jurkat cell lysates? We would like to see some validation images before ordering.

L. Zhang

Verified customer

Asked: 2019-06-05

Answer

We appreciate your inquiry. This A00421-2 anti-IL17/Il17a antibody is tested on human jurkat cell lysates, mouse spleen tissue, rat pancreas tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-05

Question

I see that the anti-IL17/Il17a antibody A00421-2 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-29

Question

I was wanting to use your anti-IL17/Il17a antibody for ELISA for rat palpebral conjunctiva on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat palpebral conjunctiva identification?

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

It shows on the product datasheet, A00421-2 anti-IL17/Il17a antibody has been tested for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat palpebral conjunctiva in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-15

Question

Is this A00421-2 anti-IL17/Il17a antibody reactive to the isotypes of IL17A?

Verified Customer

Verified customer

Asked: 2019-03-29

Answer

The immunogen of A00421-2 anti-IL17/Il17a antibody is E. coli-derived mouse IL17 recombinant protein (Position: A26-A158). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-03-29

Question

We purchased anti-IL17/Il17a antibody for ELISA on palpebral conjunctiva a few years ago. I am using human, and We are going to use the antibody for WB next. you antibody examining palpebral conjunctiva as well as t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

B. Mangal

Verified customer

Asked: 2018-01-09

Answer

I have checked the website and datasheets of our anti-IL17/Il17a antibody and I see that A00421-2 has been tested on human in both ELISA and WB. Thus A00421-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-01-09

Question

I am interested in to test anti-IL17/Il17a antibody A00421-2 on rat palpebral conjunctiva for research purposes, then I may be interested in using anti-IL17/Il17a antibody A00421-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-05-24

Answer

The products we sell, including anti-IL17/Il17a antibody A00421-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-05-24

Question

My colleagues were content with the WB result of your anti-IL17/Il17a antibody. However we have been able to see positive staining in palpebral conjunctiva secreted. using this antibody. Is that expected? Could you tell me where is IL17A supposed to be expressed?

T. Miller

Verified customer

Asked: 2014-04-03

Answer

From what I have seen in literature, palpebral conjunctiva does express IL17A. Generally IL17A expresses in secreted. Regarding which tissues have IL17A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 7499828, 14574404

Boster Scientific Support

Answered: 2014-04-03

Question

Do you have a BSA free version of anti-IL17/Il17a antibody A00421-2 available?

D. Evans

Verified customer

Asked: 2013-06-18

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL17/Il17a antibody A00421-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-06-18

Size

Conjugation

Total: $315

SKU:A00421-2

Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

Product Categories

Primary Antibodies

Primary and Secondary Antibodies

Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get a secondary antibody for free!

ELISA Kits

ELISA Kits

More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine™ ELISA kits (sandwich ELISA) and EZ-Set™ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.

Image

Recombinant Proteins

Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

Boster Kit

Reagents

Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

In stock
Order Product
A00421-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.